Complete genome sequence of a novel Bacillus phage, P59, that infects Bacillus oceanisediminis
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ANNOTATED SEQUENCE RECORD
Complete genome sequence of a novel Bacillus phage, P59, that infects Bacillus oceanisediminis Zhou Feng1 · Xinwu Liu1 · Wang Liu2 · Yong Nie1 · Xiaolei Wu1,3 Received: 19 March 2020 / Accepted: 29 June 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract P59, a virulent phage of Bacillus oceanisediminis, was isolated from the sediment of Weiming Lake at Peking University (Beijing, China). P59 showed the typical morphology of myovirids. The complete genome sequence of P59 is 159,363 bp in length with a G+C content of 42.34%. The genome sequence has very low similarity to the other phage genome sequences in the GenBank database, suggesting that P59 is a new phage. A total of 261 open reading frames and 15 tRNA genes were predicted. Based on its morphological and genetic traits, we propose phage P59 to be a new member of the family Herelleviridae. Phages are the most abundant and diverse biological entities on earth and have a great influence on global biogeochemical cycles [1]. Phages have been explored for disease treatment, industrial applications, biological research tools, and so on since the first phage was discovered over one hundred years ago [2]. With the development on culturing methods and sequencing technologies, thousands of phages have been isolated and characterized. However, isolation, sequencing and genomic analysis of novel phages are still in great need for better understanding of the phage world. Bacillus is a large and heterogeneous genus, the members of which are widely distributed in the environment [3]. This genus is also one of the most commercially exploited bacteria in industry [4]. Therefore, Bacillus phages have drawn Handling Editor: T. K. Frey. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04761-w) contains supplementary material, which is available to authorized users. * Yong Nie [email protected] * Xiaolei Wu [email protected] 1
College of Engineering, Peking University, Beijing 100871, China
2
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
3
Institute of Ocean Research, Peking University, Beijing 100871, China
the attention of many researchers [5], and a large number of phages have been isolated that use Bacillus species as their host, such as Bacillus subtilis [6], Bacillus thuringiensis [7], and Bacillus cereus [8] but no phages have been reported yet from Bacillus oceanisediminis, which was officially proposed as a new bacterial species in 2010 [9]. During an investigation of the microbial population in a subsurface oil reservoir, we isolated the strain 59-2 from oil production water sampled from Chunfeng Oilfield in China, and this strain shared 100% nucleotide sequence identity in the 16S rRNA gene with Bacillus oceanisediminis DSS-CAP-3 (GenBank ID: MF076560.1). Bacillus oceanisediminis was proposed as a novel Bacillus species in 2010 based on its unique genotypic and p
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