Characterization of Denitrifying Community for Application in Reducing Nitrogen: a Comparison of nirK and nirS Gene Dive
- PDF / 1,135,506 Bytes
- 20 Pages / 439.37 x 666.142 pts Page_size
- 68 Downloads / 165 Views
Characterization of Denitrifying Community for Application in Reducing Nitrogen: a Comparison of nirK and nirS Gene Diversity and Abundance Yingyan Wang 1 Ming Gao 1
1
& Le Qi & Rong Huang
1,2
& Fuhua Wang
1
1
& Zifang Wang &
Received: 15 August 2019 / Accepted: 8 January 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
Studies have shown that the addition of biochar to agricultural soils has the potential to mitigate climate change by decreasing nitrous oxide (N2O) emissions resulting from denitrification. Rice paddy field soils have been known to have strong denitrifying activity, but the response of microbes to biochar for weakening denitrification in rice paddy field soils is not well known. In this work, compared with the chemical fertilizer alone, the chemical fertilizer + 20 t hm−2 biochar fertilizer slightly decreased denitrifying the nitrite reductase activity (S-NiR) and N2O emission without statistic difference, whereas the chemical fertilizer + 40 t hm−2 biochar significantly boosted them. The abundance of nirdenitrifiers contributed to S-NiR and N2O emission, especially nirS-denitrifiers, rather than the variation of community structure. Pearson correlation analysis showed that NO2−-N was a key factor for controlling the abundance of nir-denitrifiers, S-NiR and N2O emission. The biochar addition fertilization treatments strongly shaped the community structure of nirK-denitrifiers, while the community structure of nirS-denitrifiers remained relatively stable. In addition, Paracoccus and Sinorhizobium were revealed to be as the predominant lineage of nirS- and nirK-containing denitrifiers, respectively. Distance-based redundancy analysis (db-RDA) showed that changes in the nir-denitrifier community structure were significantly related to soil organic carbon, NO3−-N, and total phosphorus. Our findings suggest that, although the nirS- and nirK-denitrifiers are both controlling nitrite reductase, their responses to biochar addition fertilization treatments showed significant discrepancies of diversity, abundance, and contribution to N2O and S-NiR in a paddy soil. Keywords Nir-denitrifiers . Reducing nitrogen application . Biochar . Nitrite reductase Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12010-02003250-9) contains supplementary material, which is available to authorized users.
* Zifang Wang [email protected] * Ming Gao [email protected] Extended author information available on the last page of the article
Applied Biochemistry and Biotechnology
Introduction It is well known that nitrous oxide (N2O) is a potent greenhouse gas and strongly catalyzes stratospheric ozone depletion [1]. Furthermore, recent reports have revealed that atmospheric N2O concentration has been constantly rising and reached 328 ppb in 2016 (121% of preindustrial levels), mainly due to anthropogenic intervention in the nitrogen cycle [2]. Given that agriculturally managed soils emit 4.3–5.8 Tg N2O yr−1, developing effective mitigati
Data Loading...
