Characterization of Huh7 cells after the induction of insulin resistance and post-treatment with metformin
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SHORT COMMUNICATION
Characterization of Huh7 cells after the induction of insulin resistance and post-treatment with metformin Jose´ Manuel Villalva-Pe´rez . Marco Antonio Ramı´rez-Vargas . Jesu´s Isimar Serafı´n-Fabı´an . Mo´nica Ramı´rez . Ma. Elena Moreno-Godı´nez . Mo´nica Espinoza-Rojo . Eugenia Flores-Alfaro
Received: 20 August 2019 / Accepted: 5 May 2020 Ó Springer Nature B.V. 2020
Abstract Liver-specific insulin resistance is associated with the development of the main challenges in metabolism, resulting in dyslipidemia, hyperinsulinemia and hyperglycemia. In vitro models developed for researching hepatic insulin resistance are limited and employed cell lines without similar characteristics to primary human hepatocytes. The Huh7 cell line has been established as a model with similar characteristics to primary human hepatocytes. In addition, it has been identified in the Huh7 cell line that infection with J. M. Villalva-Pe´rez J. I. Serafı´n-Fabı´an E. Flores-Alfaro (&) Laboratorio de Investigacio´n en Epidemiologı´a Clı´nica y Molecular, Facultad de Ciencias Quı´mico-Biolo´gicas, Universidad Auto´noma de Guerrero, Av. La´zaro Ca´rdenas s/n, Zip: 39089 Chilpancingo, GRO, Mexico e-mail: [email protected] M. A. Ramı´rez-Vargas Ma. Elena Moreno-Godı´nez Laboratorio de Toxicologı´a y Salud Ambiental, Facultad de Ciencias Quı´mico-Biolo´gicas, Universidad Auto´noma de Guerrero, Zip: 39087 Chilpancingo, GRO, Mexico
the hepatitis C virus induces insulin resistance. Therefore, we analyzed the induction of insulin resistance (IR) in the Huh7 cell line using an overdosage of insulin and treatment with metformin for its reversal, with the purpose of establishing an insulin resistance model useful for metabolic and pharmacological studies. Insulin-resistant Huh7 (Huh7-IR) showed a reduction in Glut2, glycogen levels, and glucose uptake stimulated by insulin or tyrosine phosphorylation from the b-fraction of insulin receptor post-insulin stimulation, with an increase of glucose production and lipid intracellular content. These biomarkers are frequently observed in insulinresistant hepatic cells. Moreover, treatment of Huh7IR with 0.5, 1 or 2 mM of metformin by 24 h decreased the biomarkers associated with an insulinresistant state. These results suggest that Huh7-IR could be used as an in vitro system to research hepatic insulin resistance in metabolic and pharmacological studies. Keywords Insulin resistance Huh7 cells Metformin Insulin resistance biomarkers
M. Ramı´rez CONACYT, Universidad Auto´noma de Guerrero, Av. Javier Me´ndez Aponte No. 1, Fracc. Servidor Agrario, Zip: 39070 Chilpancingo, GRO, Mexico
Introduction M. Espinoza-Rojo Laboratorio de Biologı´a Molecular y Geno´mica. Facultad de Ciencias Quı´mico-Biolo´gicas, Universidad Auto´noma de Guerrero, Zip: 39087 Chilpancingo, GRO, Mexico
Insulin resistance (IR) is considered the incapacity of tissues to respond to the biological action of insulin;
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Cytotechnology
this condition is associ
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