Characterization of Long Non-coding RNA Signatures of Intracranial Aneurysm in Circulating Whole Blood
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ORIGINAL RESEARCH ARTICLE
Characterization of Long Non‑coding RNA Signatures of Intracranial Aneurysm in Circulating Whole Blood Vincent M. Tutino1,2,3,4,5 · Kerry E. Poppenberg1,2 · Robert J. Damiano1,5 · Tatsat R. Patel1,5 · Muhammad Waqas1,2 · Adam A. Dmytriw9 · Kenneth V. Snyder1,2 · Adnan H. Siddiqui1,2,6 · James N. Jarvis7,8
© Springer Nature Switzerland AG 2020
Abstract Background and Objective Long non-coding RNAs (lncRNAs) may serve as biomarkers for complex disease states, such as intracranial aneurysms. In this study, we investigated lncRNA expression differences in the whole blood of patients with unruptured aneurysms. Methods Whole blood RNA from 67 subjects (34 with aneurysm, 33 without) was used for next-generation RNA sequencing. Differential expression analysis was used to define a signature of intracranial aneurysm-associated lncRNAs. To estimate the signature’s ability to classify aneurysms and to identify the most predictive lncRNAs, we implemented a nested cross-validation pipeline to train classifiers using linear discriminant analysis. Ingenuity pathway analysis was used to study potential biological roles of differentially expressed lncRNAs, and lncRNA ontology was used to investigate ontologies enriched in signature lncRNAs. Co-expression correlation analysis was performed to investigate associated differential protein-coding messenger RNA expression. Results Of 4639 detected lncRNAs, 263 were significantly different (p
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