Characterization of Macroscopic Colony-Forming Filamentous Cyanobacteria from Okinawan Coasts as Potential Sources of Bi
- PDF / 1,520,944 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 37 Downloads / 196 Views
ORIGINAL ARTICLE
Characterization of Macroscopic Colony-Forming Filamentous Cyanobacteria from Okinawan Coasts as Potential Sources of Bioactive Compounds Handung Nuryadi 1 & Shimpei Sumimoto 2 & Toshiaki Teruya 3 & Kiyotake Suenaga 4 & Shoichiro Suda 5 Received: 27 March 2020 / Accepted: 12 November 2020 / Published online: 26 November 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria are considered as prolific producers of bioactive compounds. Thus, knowledge of the diversity of MMCFF cyanobacteria as related to bioactive compound production has become very important. However, basic taxonomic studies of MMCFF cyanobacteria are lacking. Many cyanobacterial taxa are still misidentified or undescribed. In this study, a total of 32 cyanobacterial colonies from nine coastal regions of Okinawa Prefecture were investigated for a diversity assessment. A polyphasic approach including morphological and molecular studies based on 16S rRNA gene sequences was performed to characterize Okinawan MMCFF cyanobacteria. Both morphological and molecular phylogenetic results showed that MMCFF cyanobacteria from Okinawan coasts are very diverse. We found morphotypes of Lyngbya-like, Phormidium-like, and Leptolyngbya-like groups among Okinawan cyanobacterial samples. Genetically, samples were distributed in various clades in the phylogenetic tree, including within Moorena, Okeania, Caldora, Neolyngbya, Dapis, as well as several unknown clades. In addition, cytotoxic activities of three samples from Kiyan coast were tested against HeLa cells. All three crude extracts of these samples showed strong cytotoxic activity with IC50 < 1 μg/ml. Keywords Marine macroscopic colony-forming filamentous (MMCFF) cyanobacteria . Morphological characterization . 16S rRNA phylogeny . Cytotoxic activity
Introduction Cyanobacteria are well known as the first oxygenic photosynthetic bacteria (Graham and Wilcox 2000; Castenholz 2001). They play important ecological roles in global oxygen and carbon cycles, as well as in nitrogen fixation (Charpy et al. 2010, 2012). Besides those ecological roles, marine macroscopic
* Shoichiro Suda [email protected] 1
Graduate School of Engineering and Science, University of the Ryukyus, Nishihara, Okinawa, Japan
2
Department of Material and Life Chemistry, Kanagawa University, Yokohama, Kanagawa, Japan
3
Faculty of Education, University of the Ryukyus, Nishihara, Okinawa, Japan
4
Department of Chemistry, Keio University, Yokohama, Kanagawa, Japan
5
Faculty of Science, University of the Ryukyus, Nishihara, Okinawa, Japan
colony-forming filamentous (MMCFF) cyanobacteria are rich in potential secondary metabolites with biomedical properties (Nunnery et al. 2010; Tidgewell et al. 2010). In the last few decades, MMCFF cyanobacteria have become one of centers of interest in the pharmaceutical sector due to their capability to produce potential bioactive compounds. Several bioactive compounds of cyanobacteria ha
Data Loading...
