Characterization of metal element distributions in the rat brain following ischemic stroke by synchrotron radiation micr
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Characterization of metal element distributions in the rat brain following ischemic stroke by synchrotron radiation microfluorescence analysis Shu-Peng Shi1 • Hui Wang1 • Zhuo-Hui Chen1 • Xiao-Han Li1 • Shi-Xin Liu1 Meng-Qi Zhang1,2
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Received: 24 April 2020 / Revised: 16 August 2020 / Accepted: 20 August 2020 / Published online: 1 October 2020 China Science Publishing & Media Ltd. (Science Press), Shanghai Institute of Applied Physics, the Chinese Academy of Sciences, Chinese Nuclear Society and Springer Nature Singapore Pte Ltd. 2020
Abstract Ischemic stroke is one of the leading causes of death worldwide, and effective treatment strategies in the chronic phase of this disease remain insufficient. Homeostasis of metals in the brain plays an important role in maintaining normal brain function. However, the dynamic spatial distributions of iron, zinc, calcium, potassium, and copper in a rat brain following ischemic stroke and the association between structural distribution and function remain to be elucidated. In this study, we used a synchrotron radiation-based micro-X-ray fluorescence technique to image element mapping changes in special rat brain regions after ischemic stroke, showing the distribution characteristics of iron, zinc, calcium, potassium, and copper. We demonstrated, for the first time, the consistent dynamic spatial distributions of metal elements at a series of time points (3 h, 4.5 h, 6 h, 12 h, 1 d, 3 d, 5 d, 7 d, 10 d, 14 d, 28 d) after brain ischemia, which revealed that the homeostasis of iron, zinc, calcium, potassium, and copper in the brain was disturbed with distinctive change trends, providing clear insights in understanding the underlying Shu-Peng Shi, Hui Wang, and Zhuo-Hui Chen contributed equally to the work described in this paper. This work was supported by the National Natural Science Foundation of China (No. 81501025) and the Natural Science Foundation of Hunan Province (Nos. 2020JJ4134 and 2016JJ3174). & Meng-Qi Zhang [email protected] 1
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China
2
National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China
pathogenesis of stroke from a novel perspective, thus laying the foundation of further developing new drug targets for stroke treatment. Keywords Ischemic stroke Synchrotron radiation X-ray fluorescence Metal Rat brain
1 Introduction Metal elements, rich in coordination chemistry and redox properties, are effectively stored, released, and utilized by cells and tissues to form or enhance the structure and function of proteins and carry out vital life processes [1]. In the human body, the central nervous system, which is the command center for cognitive and motor functions, has immense biological complexity and contains several endogenous complexes that are involved in signaling, biosynthesis, and metabolic processes [2]. Metal elements play a particularly important role during some specific neurobiological
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