Characterization of the complete chloroplast genome of Berchemiella wilsonii var. wilsonii (Rhamnaceae), an endangered s
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TECHNICAL NOTE
Characterization of the complete chloroplast genome of Berchemiella wilsonii var. wilsonii (Rhamnaceae), an endangered species endemic to China Yin‑Huan Wang1,2 · Si‑Yun Chen1 · Shu‑Dong Zhang1
Received: 12 April 2017 / Accepted: 2 May 2017 © Springer Science+Business Media Dordrecht 2017
Abstract The complete chloroplast genome (plastome) of the endangered species Berchemiella wilsonii var. wilsonii (Rhamnaceae) was firstly reported in this study. The plastome of this species was 160,333 bp in length with a quadripartite structure that consisted of two inverted repeat (IR; 26,510 bp) regions separated by the large single-copy (LSC; 88,582 bp) and small single-copy (SSC; 18,731 bp) regions. The plastome comprised 112 unique genes including 78 protein-coding genes (PCGs), 30 tRNAs and four rRNAs. The overall GC content was 37.2%. Five types of 93 simple sequence repeats (SSRs) were detected in the plastome. The complete plastome of B. wilsonii var. wilsonii will provide valuable information for the conservation genetics and phylogenetics of Rhamnaceae. Keywords Plastome · Berchemiella wilsonii var. wilsonii · SSR · Conservation · Rhamnaceae The Rhamnaceae family consists of over 50 genera and more than 900 species, accounting for about 12% of extant Rosales diversity. Rhamnaceae is regarded as one of the most taxonomically and phylogenetically difficult taxa in plants. Rhamnaceous relationships remain unresolved in recent molecular phylogenetic studies based on a few gene loci (Richardson et al. 2000, 2004). The plastome * Shu‑Dong Zhang [email protected] 1
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China
2
Kunming College of Life Sciences, University of Chinese Academy of Sciences, Kunming 650201, Yunnan, China
has proved a valuable source of data for evaluating evolutionary relationships among angiosperms (Ruhlman and Jansen 2014). At present, however, plastomes of only one species (Ziziphus jujuba Miller) of Rhamnaceae have been published on GenBank. Berchemiella wilsonii (C. K. Schneider) Nakai, belonged to the genus of Berchemiella, family of Rhamnaceae, is an extremely endangered species listed in the China Plant Red Data Book (Fu and Jin 1992). Berchemiella wilsonii var. wilsonii is endemic to China with rare individuals restrictedly distributed in western Hubei (Kang et al. 2008). In this study, we reported and characterized the plastome of B. wilsonii var. wilsonii and detected the occurrence, type and distribution of simple sequence repeats (SSRs). This study would supply important basis for conservation genetics of B. wilsonii var. wilsonii, and promote the phylogenetic analysis of Rhamnaceae. Silica gel dried leaf materials of Berchemiella wilsonii var. wilsonii were collected from the living plant cultivated in Wuhan Botanical Garden (Hubei, China). The voucher specimen was numbered as sd206 and deposited in the herbarium of Kunming Institute of Botany, Chinese Academy of Sciences (KUN). Total ge
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