The complete chloroplast genome sequence of endangered camellias ( Camellia pubifurfuracea )
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TECHNICAL NOTE
The complete chloroplast genome sequence of endangered camellias (Camellia pubifurfuracea) Yuan Liu1,2 · Yan Han2 Received: 16 November 2017 / Accepted: 25 November 2017 © Springer Science+Business Media B.V., part of Springer Nature 2017
Abstract Camellia pubifurfuracea is endemic to Guangxi Province, China and is thought to occur in less than five locations. Moreover, its populations have been shrinking and the complete chloroplast genome has not been reported. In the present study, we report the complete chloroplast genome sequence of C. pubifurfuracea using Illumina sequencing technology. The complete chloroplast genome size is 156,975 bp. The genome contained 144 genes, including 38 tRNA genes, 100 protein-coding genes, and 6 rRNA genes. Phylogenetic analysis based on 16 complete chloroplast genomes revealed that C. pubifurfuracea is closely related to C. azalea. The chloroplast genome of C. pubifurfuracea have 54 simple sequence repeats and 50 repeat sequences, including 1 forward and 49 palindromic repeats. The complete chloroplast genome of C. pubifurfuracea will provide essential genomic information for protection the precious species of Camellia. Keywords Endangered species · Conservation · Chloroplast genome Camellia pubifurfuracea, belonging to the genus Camellia in the family Theaceae (Vijayan et al. 2009), is native in Dayao Shan (Dayao Mountain), Jinxiu County, Guangxi Province, China. It is a small tree, which grows up to 5 m tall with white flowers. It has been recorded under the evergreen forests of Mentou village (Mentoucun), and the red list category is endangered B1ab(iii) (Wheeler 2015). As compared with other Camellia species, C. pubifurfuracea has many high breeding values that grows rapidly, shade-enduring, and leanness-resistant. The new record thought to occur in less than five locations and conserving wild C. pubifurfuracea resources efficiently is a great challenge. Until now, 1790 complete chloroplast genomes have been published (https:// www.ncbi.nlm.nih.gov/genome/browse/?report=5). Part of chloroplast genomes have been used in plant biology and species identification (Kane et al. 2012; Ruhfel et al. 2014). Until date, 14 complete chloroplast genomes in the genus * Yuan Liu [email protected] 1
Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, The Chinese Academy of Sciences, Kunming 650201, China
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
2
Camellia have been reported, excluding C. pubifurfuracea (Huang et al. 2014; Shi et al. 2013; Yang et al. 2013). We assembled and analysed the complete chloroplast genome sequence of C. pubifurfuracea for the first time, to the best of our knowledge. The chloroplast genomes sequence is available the GenBank database under the accession numbers MG198673. Young leaves of C. pubifurfuracea were collected from Guangxi Province of China. Young leaves (10 g) from a single plant were used t
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