Characterization of three newly established rat sarcoma cell clones
- PDF / 476,673 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 45 Downloads / 192 Views
PORT
Characterization of three newly established rat sarcoma cell clones Monika Holubova & Martin Leba & Markéta Sedmikova & Luca Vannucci & Vratislav Horak
Received: 24 August 2012 / Accepted: 8 October 2012 / Published online: 13 November 2012 / Editor: J. Denry Sato # The Society for In Vitro Biology 2012
Abstract Establishment of new animal models using selected cell lines with different behaviour is very important for cancer investigations. In this study, we describe three morphologically distinct rat sarcoma clones—C4, C7 and D6— isolated from the R5-28 cell line. Cells of all clones expressed vimentin, fibronectin, laminin, collagen IV and matrix metalloproteinases 2 and 9. However, desmin, cytokeratins 8 and 18, ZO-1 and desmoplakins I and II were not detected. Significant proliferative capacity was documented by proliferating cell nuclear antigen expression and BrdU positivity. Karyotype of the C4, C7 and D6 cells greatly differed from diploid chromosome number of normal rat somatic cells. High expression of three cytokines—monocyte chemoattractant protein 1, tissue inhibitor of metalloproteinases 1 and vascular endothelial growth factor—was observed in all three clones. However, they varied in concentration of chemokines associated with neutrophil migration and activation—cytokine induced neutrophil chemoattractant 2 and lipopolysaccharide induced CXC chemokine. The C4 clone showed spontaneous tumour regression in vivo that was associated with significant changes in lymphocyte subpopulations. M. Holubova (*) : V. Horak Institute of Animal Physiology and Genetics, AS CR v.v.i., 277 21 Libechov, Czech Republic e-mail: [email protected] M. Holubova : M. Sedmikova Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, 160 00 Prague, Czech Republic M. Leba Faculty of Applied Science, University of West Bohemia in Pilsen, 306 14 Pilsen, Czech Republic L. Vannucci Institute of Microbiology, AS CR v.v.i., 142 20 Prague, Czech Republic
Keywords Sarcoma . Cell clones . Lewis rat . Cytokine . Spontaneous regression Sarcomas represent a heterogeneous group of tumours that can arise from various cell types of mesenchymal origin. They can be roughly divided into two main groups: bone sarcomas and soft tissue sarcomas. Various soft tissue sarcoma subtypes were described using histological, immunohistochemical and molecular criteria (Misra et al. 2009, Wibmer et al. 2010). Genetically, the soft tissue sarcomas form two groups: (a) sarcomas with specific genetic alternation used as diagnostic markers (Lazar et al. 2006) and (b) sarcomas displaying multiple, complex karyotypic abnormalities (Ladanyi and Bridge 2000, Mackall et al. 2002, Jain et al. 2010). The general incidence rate of sarcomas is low. Depending on the type, stage and age of patients, sarcomas range between one and seven cases per 100,000 persons/yr and are responsible for ∼1% of all malignancies (Wibmer et al. 2010). However, mortality is still relatively high as a result of difficulty in early diagnosis and not
Data Loading...
