Characterization of three soybean landraces resistant to Asian soybean rust disease
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Characterization of three soybean landraces resistant to Asian soybean rust disease Luciano Nobuhiro Aoyagi & Yukie Muraki & Naoki Yamanaka
Received: 19 January 2020 / Accepted: 4 May 2020 / Published online: 22 May 2020 # The Author(s) 2020
Abstract Phakopsora pachyrhizi is an obligatory biotrophic fungus that causes Asian soybean rust (ASR) disease. ASR control primarily involves chemical control and the use of resistant soybean cultivars carrying an Rpp (resistance to P. pachyrhizi) gene. This study aimed to characterize the ASR resistance of three soybean Asian landraces. By screening the world core collection (WC) of soybean, which consists of 80 varieties, three landraces were identified in Southeast Asia as resistant to ASR. Genetic mapping using the F2 population derived from a cross with an ASR-susceptible variety, BRS 184, indicated that KS 1034 (WC2) has ASR resistance conferred by a single dominant resistance gene, mapped on chromosome 18, in the same region where Rpp1 was mapped previously. The BRS 184 × WC61 (COL/THAI/1986/THAI-80) F2 population, on the other hand, showed an ASR resistance locus mapped by quantitative trait locus analysis on chromosome 6, in the region where the resistance conferred by PI 416764 Rpp3 resides, with a logarithm of the odds score peak at the same position as
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11032-020-01132-w) contains supplementary material, which is available to authorized users. L. N. Aoyagi : Y. Muraki : N. Yamanaka (*) Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan e-mail: [email protected]
the marker, Satt079, while the BRS 184 × WC51 (HM 39) population showed the resistance to ASR allocated between Satt079 and Sat_263 markers, also in the region where Rpp3 was mapped previously. Both WC51 and WC61 have the same infection profile as FT-2 and PI 462312 when tested against the same ASR isolate panel. These three WCs can be used in MAS programs for introgression of Rpp1 and Rpp3 and the development of ASR-resistant cultivars in the breeding program. Keywords Phakopsora pachyrhizi . Genetic resources . Resistance locus . Glycine max
Introduction Asian soybean rust (ASR), caused by the obligatory biotrophic Basidiomycota fungus Phakopsora pachyrhizi (Sydow & Sydow), is one of the most severe diseases affecting soybean (Glycine max), causing losses of up to 80% in ideal conditions in the various geographic regions where it has been reported, costing annually an estimated US $1.77 billion, on average (Godoy et al. 2016). Currently, the strategies for ASR management and control include the application of chemical fungicides (Embrapa 2019) and the use of specific cultivation practices, such as the elimination of secondary hosts and the introduction of soybean-free growth periods
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(sanitary periods) (Langenbach et al. 2016). Additionally, genetic resistance has been explored by developing culti
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