Cheese whey co-digestion treatment in a tubular system: microbiological behaviour along the axial axis
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ORIGINAL ARTICLE
Cheese whey co-digestion treatment in a tubular system: microbiological behaviour along the axial axis J. Jaimes-Estévez 1
&
L. Castro 1
&
H. Escalante 2
&
D. Carrillo 1,3
&
S. Portillo 1 & A. Sotres 3
&
A. Morán 3
Received: 29 June 2020 / Revised: 19 August 2020 / Accepted: 26 August 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract This study carried out an anaerobic co-digestion treatment of cheese whey using cattle manure as a co-substrate. A co-digestion process was implemented in three tubular reactors, each one with a different organic load rate (0.5, 1.0 and 1.5 kg CODfed m−3digester day−1). A biochemical and microbiological analysis along the axial axis of each reactor showed that it was possible to obtain a phase separation with an organic load rate of 1.0 kg CODfed m−3digester day−1. The volatile fatty acid production phase takes place in the first reactor section and the consumption phase in the second section. Results from highthroughput sequencing analysis showed differences in the microbial communities between the samples of the three reactors for both eubacterial and archaeal compositions. In the reactor with intermediate organic loading rate, acetoclastic communities predominate, while at lower load, both acetoclastic and hydrogenotrophic communities are detected. Additionally, a microbial consortium stratification was observed. Keywords Cattle manure . Specific methanogenic activity . High-throughput sequencing . Anaerobes
Abbreviations ACoD Anaerobic co-digestion AD Anaerobic digestion BMP Biomethane potential BPR Biogas production rate C/N Carbon/nitrogen ratio CA Correspondence analysis CM Cattle manure
CMS COD CW DNA OLR OTU PCR SBP
Cattle manure sludge Chemical oxygen demand Cheese whey Deoxyribonucleic acid Organic load rate Operational taxonomic unit Polymerase chain reaction Specific biogas production
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13399-020-00988-4) contains supplementary material, which is available to authorized users. * J. Jaimes-Estévez [email protected] L. Castro [email protected]
A. Morán [email protected] 1
Grupo de Investigación en Tecnologías de Valorización de Residuos y Fuentes Agrícolas e Industriales para la Sustentabilidad Energética (INTERFASE), Escuela de Ingeniería Química, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia
2
Centro de Estudios en Investigaciones Ambientales (CEIAM) Research Group, Escuela de Química, Universidad Industrial de Santander, Carrera 27, Calle 9, Bucaramanga, Colombia
3
Chemical, Environmental and Bioprocess Engineering Group, Universidad de León, Av. Portugal, 41, 24009 León, Spain
H. Escalante [email protected] D. Carrillo [email protected] S. Portillo [email protected] A. Sotres [email protected]
Biomass Conv. Bioref.
SMA SP SPP TA TR VFA VS
Specific methanogenic activity Sampling port Struvite precipitation potential Total alkalinity Tubular reactor Volatile fatty ac
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