Chemical Composition of Ethanolic Extracts from some Seaweed Species of the South Indian Coastal Zone, their Antibacteri
- PDF / 1,122,166 Bytes
- 9 Pages / 612 x 792 pts (letter) Page_size
- 23 Downloads / 187 Views
RIGINAL PAPERS
Chemical Composition of Ethanolic Extracts from some Seaweed Species of the South Indian Coastal Zone, their Antibacterial and Membrane-Stabilizing Activity1 Boobalan Bharatha, Arun Nagarajan Pavithraa, Arumugam Divyaa, and Kantharaj Perinbama, * a
PG and Research Department of Botany, Government Arts College for Men, Affiliated to University of Madras, Nandanam, Chennai, Tamil Nadu, 600035 India *e-mail: [email protected] Received November 20, 2019; revised March 18, 2020; accepted March 19, 2020
Abstract—The present study was intended to investigate the presence of major chemical components in seaweed extracts by using of Fourier-transform infrared spectroscopy (FTIR) and to explore the antibacterial and in vitro anti-inflammatory (membrane stability) properties of ethanolic extracts of nine different seaweeds collected from the Ramewaram coastal region. Total flavonoid content (TFC) and total tannin content (TTC) of ethanolic extracts were determined using spectrometric method. Antimicrobial activity of ethanolic extracts was tested against six bacterial strains using the disc diffusion method. Study of the preliminary antiinflammatory activity of seaweed extracts was performed by the HRBC membrane stabilization method. Turbinaria ornata shows the highest TFC and TTC among all the studied seaweed species. Significant differences were observed in anti-inflammatory activity of ethanolic extracts from various seaweeds investigated using the human red blood cell (HRBC) membrane stabilization method. HRBC membrane stabilization activity of ethanolic extracts of seaweeds exhibited dose dependency and increased with increasing concentration of the extract. Ethanolic extract of T. ornata had the strongest growth inhibition against Proteus mirabilis. FTIR spectroscopy analysis of three seaweeds provided a detailed report of major functional constituents and chemical components. It can be concluded that seaweeds are rich in polyphenols to which acquire potent antimicrobial and anti-inflammatory activity. Seaweed extracts are a major breakthrough for pharmaceutical application. Keywords: seaweeds, flavonoid, tannin, antimicrobial, anti-inflammatory, FTIR DOI: 10.1134/S1063074020050041
INTRODUCTION Natural products from marine environment, particularly macroalgae, represent extensive therapeutic drugs for various diseases [18]. Generally, seaweeds are classified based on their pigmentation into three broad groups, namely green algae (Chlorophyceae), brown algae (Phaeophyceae), and red algae (Rhodophyceae) [19]. The coastlines of south India, especially Rameswaram, are rich in seaweed diversity with broad species. Recent reviews of seaweed extracts focus on seaweed liquid fertilizers, antioxidant, anticancer, anticoagulant, anti-inflammatory, antiviral, anti-arthritic, antiulcer etc., various complex mixtures of bioactive compounds such as polyphenols, proteins, polysaccharides, peptides, terpenoids, polyunsaturated fatty acids (PUFA), plant growth hormones, vitamins, minerals, and pigments [2, 11, 30,
Data Loading...
