Chimeric Newcastle Disease Virus-like Particles Containing DC-Binding Peptide-Fused Haemagglutinin Protect Chickens from
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RESEARCH ARTICLE
Chimeric Newcastle Disease Virus-like Particles Containing DC-Binding Peptide-Fused Haemagglutinin Protect Chickens from Virulent Newcastle Disease Virus and H9N2 Avian Influenza Virus Challenge Xiaohong Xu1,2 • Jing Qian3 • Lingsong Qin1 • Jindou Li1 • Cong Xue4 • Jiaxin Ding1 • Weiqi Wang1 Wei Ding1 • Renfu Yin1 • Ningyi Jin2 • Zhuang Ding1
•
Received: 3 February 2019 / Accepted: 18 November 2019 Ó Wuhan Institute of Virology, CAS 2020
Abstract Newcastle disease virus (NDV) and H9N2 subtype Avian influenza virus (AIV) are two notorious avian respiratory pathogens that cause great losses in the poultry industry. Current inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses, while an attenuated live vaccine bears the risk of mutation. Dendritic cell (DC) targeting strategies are attractive for their potent mucosal and adaptive immune-stimulating ability against respiratory pathogens. In this study, DC-binding peptide (DCpep)-decorated chimeric virus-like particles (cVLPs), containing NDV haemagglutinin–neuraminidase (HN) and AIV haemagglutinin (HA), were developed as a DC-targeting mucosal vaccine candidate. DCpep-decorated cVLPs activated DCs in vitro, and induced potent immune stimulation in chickens, with enhanced secretory immunoglobulin A (sIgA) secretion and splenic T cell differentiation. 40 lg cVLPs can provide full protection against the challenge with homologous, heterologous NDV strains, and AIV H9N2. In addition, DCpep-decorated cVLPs could induce a better immune response when administered intranasally than intramuscularly, as indicated by robust sIgA secretion and a reduced virus shedding period. Taken together, this chimeric VLPs are a promising vaccine candidate to control NDV and AIV H9N2 and a useful platform bearing multivalent antigens. Keywords Newcastle disease virus-like particles DC-binding peptide H9N2 Secretory immunoglobulin A (sIgA) Candidate vaccine
Introduction & Renfu Yin [email protected] & Ningyi Jin [email protected] & Zhuang Ding [email protected] 1
Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China
2
College of Basic Medical Science, Jilin University, Changchun 130021, China
3
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory for Veterinary BioProduct Engineering, Ministry of Agriculture, Nanjing 210014, China
4
College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China
Newcastle disease virus (NDV) and avian influenza virus (AIV) are among the most notorious avian pathogens listed by The World Organisation for Animal Health (OIE) (OIE 2012). The infection of the two viruses causes severe economic losses in the poultry industry worldwide. The AIV strain H9N2 normally causes depression, ruffled feathers, and body weight loss in chic
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