Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis
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Cancer Cell International Open Access
PRIMARY RESEARCH
Circ_0000285 regulates proliferation, migration, invasion and apoptosis of osteosarcoma by miR‑409‑3p/IGFBP3 axis Zhisheng Long, Feipeng Gong, Yuxu Li, Zhiqiang Fan and Jingtang Li*
Abstract Background: Circular RNAs (circRNAs) are important regulators in the pathogenesis of diseases and affects the occurrence and development of diseases. However, the role of circRNAs in osteosarcoma (OS) has not been fully elucidated. Methods: The expression of circ_0000285, miR-409-3p and insulin-like growth factor binding protein 3 (IGFBP3) was detected using quantitative real-time PCR (qRT-PCR). The protein level of IGFBP3 was measured using western blot. CCK-8 and colony formation assays were used to determine cell proliferation. Flow cytometry was applied to measure cell cycle and cell apoptosis. Transwell assay was used to assess cell invasion and migration. Dual-luciferase reporter assay and RNA Binding Protein Immunoprecipitation (RIP) assay were performed to determine the relationship among circ_0000285, miR-409-3p and IGFBP3. The animal experiments were performed to determine the function of circ_0000285 in vivo. Results: In this study, we found that the expression of circ_0000285 was significantly increased in OS tissues and cells and was enriched in the cytoplasm. Knockdown of circ_0000285 inhibited OS growth in vitro and in vivo. Moreover, miR-409-3p was a target miRNA of circ_0000285 and miR-409-3p targets to IGFBP3 in OS. Besides, circ_0000285 could promote proliferation, migration, invasion and inhibit apoptosis of osteosarcoma by miR-409-3p/IGFBP3 axis. Conclusion: In this study, circ_0000285 regulated proliferation, migration, invasion and apoptosis of OS cells by miR409-3p/IGFBP3 axis, implying that circ_0000285 was a potential target for OS therapy. Keywords: circ_0000285, miR-409-3p, IGFBP3, Cell growth, OS Highlights 1. circ_0000285 expression was enhanced in osteosarcoma tissues and cells. 2. Suppression of circ_0000285 inhibited osteosarcoma progression in vitro and in vivo.
*Correspondence: [email protected] Department of Orthopaedics, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, No.92, AiGuo Road, Nanchang City 330006, Jiangxi Province, China
3. circ_0000285 targeting miR-409-3p to upregulate IGFBP3, and consequently regulates proliferation, migration, invasion and apoptosis of osteosarcoma.
Background Osteosarcoma (OS) is a type of malignant tumor with a high incidence in adolescents and about 60% of OS patients are under 25 years of age [1–3]. The cause and mechanism of osteosarcoma are still unclear, which make it difficult to diagnose and treat osteosarcoma. Cirular RNA (circRNA) is a special type of non-coding RNA with closed-loop structure and its expression
© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the ori
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