MiR-455-3p downregulation facilitates cell proliferation and invasion and predicts poor prognosis of osteosarcoma
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(2020) 15:454
RESEARCH ARTICLE
Open Access
MiR-455-3p downregulation facilitates cell proliferation and invasion and predicts poor prognosis of osteosarcoma Xijun Yi1, Yafei Wang2 and Shijie Xu3*
Abstract Background: Osteosarcoma (OS) is one of the most primary malignant bone tumors, mainly attracting children and young adults. The microRNAs are mentioned to play vital roles in many cancers, including OS. The purpose of this study was to explore the expression and function of miR-455-3p in OS and predict the potential effects in clinical diagnosis and prognosis. Method: We conducted quantitative real-time PCR to assess the expression of miR-455-3p in OS tissues and cell lines. The Cell Counting Kit-8 assay, Transwell assay, and flow cytometry were performed to assess the ability of miR-455-3p on cell proliferation, migration, invasion, and apoptosis. Kaplan–Meier curve and Cox regression analysis were used to demonstrate the survival outcome. Results: This study revealed that the expression of miR-455-3p was decreased in OS tissues and cell lines. The dysregulation of miR-455-3p was in association with tumor size, distant metastasis, and clinical stage. Patients with high miR-455-3p expression had a satisfying survival rate. Multivariate Cox analysis indicated that miR-455-3p was a promising prognostic indicator. Expression of miR-455-3p could inhibit the proliferation, migration, and invasion, and facilitate apoptosis of OS cells in vitro. Conclusion: These results indicated the miR-455-3p was a potential clinical therapeutic target and prognostic biomarker by suppressing the proliferation, migration, and invasion, as well as enhancing cell apoptosis. Keywords: Osteosarcoma, miR-455-3p, Prognosis, Proliferation, Migration, Invasion
Background Osteosarcoma (OS) is the most common malignant tumor worldwide among children and adulthood [1]. The annual incidence of OS is 2–3 million, but is higher in adolescence, in which the annual incidence peaks at 8–11 million at 15–19 years of age [2]. The etiology of OS is heterogeneous and unclear. It is commonly recognized that genetic mutation [3], various demographics [4], and environmental factors [5] associate with OS. * Correspondence: [email protected] 3 Second Department of Traumatology, Linyi People’s Hospital, Linyi 276000, Shandong, China Full list of author information is available at the end of the article
Treatment for patients with OS involves surgical resection in conjunction with systemic chemotherapy—neoadjuvant and adjuvant, unfortunately, the outcome has no improvement for several decades [6, 7]. Thus, novel prognostic indicators are crucial for the amelioration of OS therapy. MicroRNAs (miRNAs) are a class of small non-coding RNAs that restrict their targets by binding to the 3′UTR and/or coding sequences [8]. Numerous studies have shown that miRNAs play important roles in the diagnosis, treatment, and prognosis of OS patients [9]. A study conducted by Liu et al. shows that the miR-29 is upregulated in OS and promotes cell proliferation and
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