Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3
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RESEARCH
Open Access
Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR4677-3p/CDCA3 Koudong Zhang1, Hang Hu1, Juan Xu1, Limin Qiu2, Haitao Chen3, Xingzhi Jiang1 and Yongqian Jiang1*
Abstract Background: Lung cancer (LC) is a malignant tumor originating in the bronchial mucosa or gland of the lung. Circular RNAs (circRNAs) are proved to be key regulators of tumor progression. However, the regulatory effect of circ_0001421 on lung cancer tumorigenesis remains unclear. Methods: The expression levels of circ_0001421, microRNA-4677-3p (miR-4677-3p) and cell division cycle associated 3 (CDCA3) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Methyl thiazolyl tetrazolium (MTT), Transwell and Tumor formation assays were performed to explore the role of circ_0001421 in LC. Glucose consumption and lactate production were examined by a Glucose assay kit and a Lactic Acid assay kit. Western blot was utilized to examine the protein levels of Hexokinase 2 (HK2) and CDCA3. The interaction between miR-4677-3p and circ_0001421 or CDCA3 was confirmed by dual-luciferase reporter assay. Results: Circ_0001421 was increased in LC tissues and cells, and knockdown of circ_0001421 repressed cell proliferation, migration, invasion and glycolysis in vitro. Meanwhile, circ_0001421 knockdown inhibited LC tumor growth in vivo. Mechanistically, circ_0001421 could bind to miR-4677-3p, and CDCA3 was a target of miR-4677-3p. Rescue assays manifested that silencing miR-4677-3p or CDCA3 overexpression reversed circ_0001421 knockdownmediated suppression on cell proliferation, migration, invasion and glycolysis in LC cells. Conclusion: Circ_0001421 promoted cell proliferation, migration, invasion and glycolysis in LC by regulating the miR-4677-3p/CDCA3 axis, which providing a new mechanism for LC tumor progression. Keywords: Lung cancer, circ_0001421, miR-4677-3p, CDCA3
Background Lung cancer (LC) is a malignant tumor with the highest morbidity and mortality in worldwide [1, 2]. According to histopathological classification, LC included non-small cell lung cancer (NSCLC) and small cell lung cancer. The 5year survival rate of LC patients is only 17.7%, mainly on account of the rapid tumor metastasis and local recurrence [3]. Besides, the 5-year survival rate of patients with * Correspondence: [email protected] 1 Department of Respiratory and Critical Medicine, The Fourth Affiliated Hospital of Nantong University; The First People’s Hospital of Yancheng, No.66 Renmin South Road, Yancheng 224000, Jiangsu, China Full list of author information is available at the end of the article
early-stage LC is significantly higher than that of advanced LC patients [4]. Hence, it is necessary to dive deep into the underlying mechanism of lung cancer tumorigenesis and progression. Circular RNA (circRNA) is a special form of non-coding RNA (ncRNA) that form a loop with jointed 3′ heads and 5′ tails [5]. CircRNAs have received attention due to their conservation and tissue specific expression in living bein
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