Circ0120816 acts as an oncogene of esophageal squamous cell carcinoma by inhibiting miR-1305 and releasing TXNRD1
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Cancer Cell International Open Access
PRIMARY RESEARCH
Circ0120816 acts as an oncogene of esophageal squamous cell carcinoma by inhibiting miR‑1305 and releasing TXNRD1 Xiaoyong Li, Laichun Song, Bo Wang, Chao Tao, Lei Shi and Ming Xu*
Abstract Background: Circular RNAs (circRNAs) have been discovered to participate in the carcinogenesis of multiple cancers. However, the role of circRNAs in esophageal squamous cell carcinoma (ESCC) progression is yet to be properly understood. This research aimed to investigate and understand the mechanism used by circRNAs to regulate ESCC progression. Methods: Bioinformatics analysis was first performed to screen dysregulated circRNAs and differentially expressed genes in ESCC. The ESCC tissue samples and adjacent normal tissue samples utilized in this study were obtained from 36 ESCC patients. All the samples were subjected to qRT-PCR analysis to identify the expression of TXNRD1, circRNAs, and miR-1305. Luciferase reporter assay, RNA immunoprecipitation assay and RNA pull-down assay were later conducted to verify the existing relationship among circ0120816, miR-1305 and TXNRD1. CCK-8, BrdU, cell adhesion, cell cycle, western blot and caspase 3 activity assays were also employed to evaluate the regulation of these three biological molecules in ESCC carcinogenesis. To evaluate the effect of circ0120816 on ESCC tumor growth and metastasis, the xenograft mice model was constructed. Results: Experimental investigations revealed that circ0120816 was the highest upregulated circRNA in ESCC tissues and that this non-coding RNA acted as a miR-1305 sponge in enhancing cell viability, cell proliferation, and cell adhesion as well as repressing cell apoptosis in ESCC cell lines. Moreover, miR-1305 was observed to exert a tumorsuppressive effect in ESCC cells by directly targeting and repressing TXNRD1. It was also noticed that TXNRD1 could regulate cyclin, cell adhesion molecule, and apoptosis-related proteins. Furthermore, silencing circ0120816 was found to repress ESCC tumor growth and metastasis in vivo. Conclusions: This research confirmed that circ0120816 played an active role in promoting ESCC development by targeting miR-1305 and upregulating oncogene TXNRD1. Keywords: circ0120816, miR-1305, TXNRD1, Esophageal squamous cell carcinoma
*Correspondence: [email protected] Department of Cardiac Surgery, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan Asia Heart Hospital Affiliated to Wuhan University of Science and Technology, No.753 Jinghan Road, Wuhan 430022, Hubei, China
Background Esophageal cancer (EC) refers to the malignant growth found in the hollow tube connecting the throat to the stomach. In 2018, 572,034 new cases and 508,585 deaths were associated with EC worldwide [1]. Among them, 90% were identified as esophageal squamous cell carcinoma (ESCC) [2]. The incidence of ESCC was discovered to be extremely high in China: It accounted for about 50% of EC recorded globally [3, 4]. In the last 10 years,
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