Coastal bacterioplankton community diversity along a latitudinal gradient in Latin America by means of V6 tag pyrosequen
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ORIGINAL PAPER
Coastal bacterioplankton community diversity along a latitudinal gradient in Latin America by means of V6 tag pyrosequencing Fabiano L. Thompson · Thiago Bruce · Alessandra Gonzalez · Alexander Cardoso · Maysa Clementino · Marcela Costagliola · Constanza Hozbor · Ernesto Otero · Claudia Piccini · Silvia Peressutti · Robert Schmieder · Robert Edwards · Mathew Smith · Luis Roberto Takiyama · Ricardo Vieira · Rodolfo Paranhos · Luis Felipe Artigas
Received: 25 May 2010 / Revised: 26 August 2010 / Accepted: 4 October 2010 / Published online: 13 November 2010 © Springer-Verlag 2010
Abstract The bacterioplankton diversity of coastal waters along a latitudinal gradient between Puerto Rico and Argentina was analyzed using a total of 134,197 high-quality sequences from the V6 hypervariable region of the small-subunit ribosomal RNA gene (16S rRNA) (mean length of 60 nt). Most of the OTUs were identiWed into Proteobacteria, Bacteriodetes, Cyanobacteria, and Actinobacteria, corresponding to approx. 80% of the total number
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (doi:10.1007/s00203-010-0644-y) contains supplementary material, which is available to authorized users. F. L. Thompson (&) · T. Bruce Departments of Genetics, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil e-mail: [email protected] A. Gonzalez · R. Paranhos Departments of Marine Biology, Institute of Biology, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil A. Cardoso · R. Vieira Institute of Medical Biochemistry, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil M. Clementino Institute of Quality Control in Health, Department of Microbiology FIOCRUZ, Rio de Janeiro, Brazil R. Schmieder · R. Edwards Department of Computer Science and Computational Science Research Center, San Diego State University, San Diego, CA, USA
of sequences. The number of OTUs corresponding to species varied between 937 and 1946 in the seven locations. Proteobacteria appeared at high frequency in the seven locations. An enrichment of Cyanobacteria was observed in Puerto Rico, whereas an enrichment of Bacteroidetes was detected in the Argentinian shelf and Uruguayan coastal lagoons. The highest number of sequences of Actinobacteria and Acidobacteria were obtained in the Amazon estuary mouth. The rarefaction curves and Good coverage estimator for species diversity suggested a signiWcant coverage, with values ranging between 92 and 97% for Good coverage. Conserved taxa corresponded to aprox. 52% of all sequences. This study suggests that human-
M. Costagliola · C. Hozbor · S. Peressutti Instituto Nacional de Investigación y Desarrollo Pesquero (INIDEP), Mar del Plata, Argentina E. Otero · M. Smith Departamento de Ciencias Marinas, Universidad de Puerto Rico, Mayagüez, PR, USA C. Piccini Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, 11600 Montevideo, Uruguay L.
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