Comparative analysis of genomic- and EST-SSRs in European plum ( Prunus domestica L.): implications for the diversity an
- PDF / 1,313,786 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 84 Downloads / 175 Views
ORIGINAL ARTICLE
Comparative analysis of genomic‑ and EST‑SSRs in European plum (Prunus domestica L.): implications for the diversity analysis of polyploids Rosanna Manco1 · Pasquale Chiaiese1 · Boris Basile1 · Giandomenico Corrado1 Received: 16 April 2020 / Accepted: 27 October 2020 / Published online: 21 November 2020 © The Author(s) 2020
Abstract Simple sequence repeats (SSRs) are among the most useful DNA markers in plant science. The aim of this study was to compare the features and usefulness of genomic SSR (gSSR) and EST-SSRs in European plum (Prunus domestica L.), an economically important, hexaploid stone fruit crop globally cultivated to produce fleshy fruits and derived foodstuff. The analysis of an ample set of morphologically diverse varieties indicated that gSSRs and EST-SSRs provide different estimates of some of the locus-based indicators of diversity. Moreover, the two classes of SSRs gave different, weakly correlated, estimations of distance-based parameters with gSSRs being more powerful for discriminating purposes. The two SSR classes provide complementary information in European plum, making the contribution of EST-SSRs useful not only as non-neutral markers. The differences between SSR classes are discussed considering the neutral and non-neutral evolution, and the polyploidy and asexual propagation of the cultivated tree varieties. Keywords Stone fruit · Polyploidy · Polymorphism · Diversity · Microsatellites
Introduction European plum (Prunus domestica L.) is an economically important stone fruit crop, globally cultivated in temperate areas for its fleshy fruits. These are mainly marketed fresh, canned or dried (Neumüller 2011). Further uses include the production of juices, fruit brandy, and flavours for jams, candies, sweets, and other baked foods. Commercially available European plum varieties present a range of phenotypic traits (e.g., fruits shape and size; skin and flesh colour; firmness; taste) that originated several classifications. Cultivated varieties are typically classified in different pomological groups by breeders and retailers, although the degree of overlap of morphological traits and the complex interspecific origin of
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02513-w) contains supplementary material, which is available to authorized users. * Giandomenico Corrado [email protected] 1
Dipartimento di Agraria, Università Degli Studi di Napoli Federico II, via Università 100, 80055 Portici, NA, Italy
the species do not always allow a clear distinction among all the different groups (Zhebentyayeva et al. 2019). Considerable progress in describing and classifying the ample diversity of the European plum has been achieved with the introduction of DNA molecular markers (Decroocq et al. 2004; Dirlewanger et al. 2002; Li et al. 2010; Shimada et al. 1999). These analyses also confirmed that the European plum clade of P. domestica has a high level of diversity (Zhebentyayeva et al. 2019). Moreove
Data Loading...
