Comparative analysis of the gut microbiota of Apis cerana in Yunnan using high-throughput sequencing
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ORIGINAL PAPER
Comparative analysis of the gut microbiota of Apis cerana in Yunnan using high‑throughput sequencing Zhi‑Wen Luo1 · Zhi‑Xiang Dong1 · Yi‑Fei Chen1 · Huan‑Yuan Li1 · Qi‑He Tang1 · Ji‑Lian Li2 · Jun Guo1 Received: 21 August 2019 / Revised: 30 April 2020 / Accepted: 3 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Gut microbes play an important role in host disease and health. The Asian honey bee Apis cerana is an important pollinator of agricultural crops in China. However, there are still few studies on the structure and composition of the microbiota in the intestine of A. cerana, especially A. cerana in Yunnan. To understand the species and composition of the microbiota in the intestine of A. cerana in Yunnan, we used high-throughput sequencing technology to carry out 16S rRNA sequencing on 50 samples from Kunming, Xishuangbanna and Mengzi. The results show that both from the phylum level and the genus level, the structure and abundance of the microbiota in the gut of A. cerana from the three regions tended to be the same. At the phylum level, the abundance of Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, Acidobacteria and other species was high in A. cerana from different areas. At the genus level, the abundance of Lactobacillus, Gilliamella, Snodgrassella, Apibacter, Candidatus Schmidhempelia and other species was high in A. cerana from different areas. Due to its unique geographical environment and climatic conditions, at the genus level, the diversity of bacterial communities in Xishuangbanna was significantly lower than that in the other two regions, which was about 100 genera less. In conclusion, our results reveal the composition and structure of the intestinal microbiota of bees in Yunnan and deepen our understanding of the intestinal microbiota of bees. Keywords Gut microbes · Apis cerana · Microbiota structure · 16S rRNA sequencing
Introduction Apis cerana belongs to the honeybee family and the honeybee genus, which is a subspecies of Asian honeybees, and it is a unique bee species in China. As an important pollinator insect, this bee plays an important role in pollinating plants (Crowther et al. 2019), maintaining ecological balance and Communicated by Erko Stackebrandt. Zhi-Wen Luo and Zhi-Xiang Dong contributed equally. * Ji‑Lian Li [email protected] * Jun Guo [email protected] 1
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, Yunnan, China
Institute of Apiculture, Chinese Academy of Agricultural Science/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Beijing 100093, China
2
plant species diversity, and improving crop yield and quality. A. cerana is widely distributed in China, and the Yunnan–Guizhou plateau and austroyunnanese are the central production areas of A. cerana. Kunming, Xishuangbanna and Mengzi are important breeding areas (Luo and Chen 2018). Due to the maturity of local bee breeding technology, the bee industry has developed and has not
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