Investigation of the gut microbiome of Apis cerana honeybees from Vietnam
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ORIGINAL RESEARCH PAPER
Investigation of the gut microbiome of Apis cerana honeybees from Vietnam Bui Thi Thuy Duong . Nguyen Thi Kim Lien . Ha Thi Thu . Nguyen Thi Hoa . Pham Thi Lanh . Bo-Ram Yun . Mi-Sun Yoo . Yun Sang Cho . Dong Van Quyen
Received: 24 February 2020 / Accepted: 19 June 2020 Ó Springer Nature B.V. 2020
Abstract Objectives In this study, the gut microbiome of healthy adult honeybees, Apis cerana, was investigated by sequencing the V3 – V4 region in 16S rRNA gene using Illumina Miseq platform. Results The total of 37,853 reads for 16S rRNA gene were obtained and 30,121 (79.6%) reads were valid with 25,291 (84.0%) reads that were classified into 116 species belonging to four major phyla. The relative abundances of the bacterial isolates in honeybee samples were phylum Proteobacteria (70.7%), Actinobacteria (10.7%), Firmicutes (10.3%), and Bacteroidetes (8.4%), respectively. Lactic acid
bacteria comprised 18.95% with 10 groups including Bifidobacterium asteroides, B. indicum, Fructobacillus fructosus, Lactobacillus apinorum, L. apis, L. helsingborgensis, L. kimbladii, L. kullabergensis, and L. kunkeei. Conclusions The presence of beneficial bacteria in the gut highlighted their role in the honeybee and suggested that they can be promising candidates for the development of probiotics for health improvement, infection control and disease management of honeybees. Keywords Apis cerana Honeybee 16S rRNA sequence Microbiome Vietnam
Bui Thi Thuy Duong and Nguyen Thi Kim Lien (co-first authors) have contributed equally to this work. B. T. T. Duong H. T. Thu N. T. Hoa P. T. Lanh D. Van Quyen Molecular Microbiology Lab, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam
D. Van Quyen (&) University of Science and Technology of Ha Noi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam e-mail: [email protected]; [email protected]
N. T. K. Lien Functional Genomics Lab, Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam B.-R. Yun M.-S. Yoo Y. S. Cho (&) Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, 39660 Gimcheon-si, Gyeongsangbuk-do, Republic of Korea e-mail: [email protected]
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Biotechnol Lett
Abbreviations AMPs Antimicrobial peptides DNA Deoxyribonucleic acid EDTA Ethylene diamine tetraacetic acid NGS Next generation sequencing OTU Operational taxonomic unit PCR Polymerase chain reaction QC Quality check rRNA Ribosomal ribonucleic acid SDS Sodium d¯oecyl sulfate
Introduction Beekeeping provides people the high-value products such as honey, pollen, propolis, bee venom and contributes great profit to beekeepers. Improving the health of honeybees has always been the target of many researches. Recent studies have shown that the gut microbiota of honeybees plays an important role in improving the honeybees’ health (Evans and Schwarz 2011
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