Comparative Evaluation of RTi-PCR and Mini-VIDAS SLM System as Predictive Tools for the Routine Detection of Salmonella
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Comparative Evaluation of RTi-PCR and Mini-VIDAS SLM System as Predictive Tools for the Routine Detection of Salmonella spp. in Naturally Contaminated Food Products P. Elizaquível & J. A. Gabaldón & R. Aznar
Received: 16 April 2008 / Accepted: 30 June 2008 / Published online: 1 August 2008 # Springer Science + Business Media, LLC 2008
Abstract In the present work, we have evaluated a realtime polymerase chain reaction-based method (RTi-PCR) as a routine laboratory test, by comparing the results obtained in two laboratories with the mini-VIDAS SLM (bioMérieux), a standard accepted immunoenzymatic (enzyme-linked immunosorbent assay) method currently used in the food industry. To that aim, a set of 141 naturally contaminated food samples were analyzed after an enrichment step by conventional PCR and mini-VIDAS and prior to the enrichment by RTi-PCR. Results from both laboratories were statistically analyzed using the kappa coefficients, which indicated a perfect agreement between them. Out of the 141 samples, 11 were positive for Salmonella detection by mini-VIDAS, 35 by conventional PCR in Lab1, and 44 in Lab2. Twentyeight of them tested positive by RTi-PCR in Lab1 and 31 in Lab2. In order to use the evaluated methods as a diagnostic test, their predictive capacity was analyzed on the basis of their positive and negative predictive values, calculated using the result obtained—after enrichment—by conventional PCR as the “gold standard.” Both positive and P. Elizaquível : R. Aznar Departamento de Microbiología y Ecología, Universitat de València, Valencia, Spain J. A. Gabaldón Centro Tecnológico Nacional de la Conserva y Alimentación, Departamento de Seguridad Alimentaria, Murcia, Spain R. Aznar (*) Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Apartado de Correos 73, 46100 Burjassot, Valencia, Spain e-mail: [email protected]
negative predictive values were higher for RTi-PCR than those obtained for mini-VIDAS, indicating good performance of the RTi-PCR technique—applied without enrichment—in the detection of Salmonella in food products. Overall, results obtained in this study on naturally contaminated food products highlighted that RTi-PCR without enrichment is a better predictive tool than mini-VIDAS. Keywords Salmonella spp. . Mini-VIDAS SLM . PCR . RTi-PCR . Food
Introduction Salmonella spp. is one of the most common and widely distributed foodborne pathogens. It constitutes a major public health burden and represents a significant cost in many countries (WHO 2005). The reservoir for this pathogen is mainly the gastrointestinal tract of mammals and birds often adopting asymptomatic infection (Bäumler et al. 2000; Guard-Petter 2001). Consumption of contaminated eggs, meat, and meat products is the main cause for salmonellosis outbreaks. However, in recent years, the growing consumption of ready-to-eat products has lead to an increase in the number of salmonellosis outbreaks linked to different kinds of food products including fres
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