Comparative transcriptomic analysis reveals the mechanistic basis of Pib -mediated broad spectrum resistance against Mag
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ORIGINAL ARTICLE
Comparative transcriptomic analysis reveals the mechanistic basis of Pib-mediated broad spectrum resistance against Magnaporthe oryzae Jiehua Qiu 1 & Feifei Lu 1 & Meng Xiong 2 & Shuai Meng 1 & Xianglin Shen 2 & Yanjun Kou 1 Received: 29 July 2019 / Revised: 11 August 2020 / Accepted: 27 August 2020 # The Author(s) 2020
Abstract Rice blast, caused by the fungus Magnaporthe oryzae, is a highly damaging disease. Introducing genes, which confer a broad spectrum resistance to the disease, such as Pib, makes an important contribution to protecting rice production. However, little is known regarding the mechanistic basis of the products of such genes. In this study, transcriptome of the cultivar Lijiangxintuanheigu (LTH) and its monogenic IRBLb-B which harbors Pib treated with M. oryzae were compared. Among the many genes responding transcriptionally to infection were some encoding products involved in the metabolism of ROS (reactive oxygen species), in jasmonate (JA) metabolism, and WRKY transcription factors, receptor kinases, and resistance response signal modulation. The down-regulation of genes encoding peroxiredoxin and glutathione S transferases implied that the redox homeostasis is essential for the expression of Pibmediated resistance. The up-regulation of seven disease resistance-related genes, including three encoding a NBS-LRR protein, indicated that disease resistance-related genes are likely tend to support the expression of Pib resistance. These data revealed that potential candidate genes and transcriptional reprogramming were involved in Pib-mediated resistance mechanisms. Keywords Magnaporthe oryzae . Resistant near isogenic line . RNA-seq . Broad-spectrum resistance . Pib . Redox
Introduction Rice is a staple food for half of the world’s population. Like all crops, its production is constrained by a number of both biotic and abiotic stresses. Among the various stresses, rice blast, which is caused by fungal pathogen Magnaporthe oryzae, is the most devastating disease in rice production. It occurs
almost in all rice planting areas, generally resulting in rice yield loss of 10 to 30% annually (Dean et al. 2012). An increase in understanding the molecular mechanisms of the rice–M. oryzae interaction may be useful to develop novel strategies for blast control. To resist pathogen invasion, plants have developed a complex system of defense against microbial pathogens such as
Jiehua Qiu and Feifei Lu contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10142-020-00752-x) contains supplementary material, which is available to authorized users. * Yanjun Kou [email protected]
Shuai Meng [email protected]
Jiehua Qiu [email protected] Feifei Lu [email protected] Meng Xiong [email protected]
Xianglin Shen [email protected] 1
State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
2
Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement
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