Comparison of Different Lactobacilli Regarding Substrate Utilization and Their Tolerance Towards Lignocellulose Degradat
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Comparison of Different Lactobacilli Regarding Substrate Utilization and Their Tolerance Towards Lignocellulose Degradation Products Angela Gubelt1,2 · Lisa Blaschke1,3 · Thomas Hahn4 · Steffen Rupp1,4 · Thomas Hirth1,4,5 · Susanne Zibek1,4 Received: 2 March 2020 / Accepted: 14 July 2020 © The Author(s) 2020
Abstract Fermentative lactic acid production is currently impeded by low pH tolerance of the production organisms, the successive substrate consumption of the strains and/or the requirement to apply purified substrate streams. We identified Lactobacillus brevis IGB 1.29 in compost, which is capable of producing lactic acid at low pH values from lignocellulose hydrolysates, simultaneously consuming glucose and xylose. In this study, we compared Lactobacillus brevis IGB 1.29 with the reference strains Lactobacillus brevis ATCC 367, Lactobacillus plantarum NCIMB 8826 and Lactococcus lactis JCM 7638 with regard to the consumption of C5- and C6-sugars. Simultaneous conversion of C5- and C6-monosaccharides was confirmed for L. brevis IGB 1.29 with consumption rates of 1.6 g/(L h) for glucose and 1.0 g/(L h) for xylose. Consumption rates were lower for L. brevis ATCC 367 with 0.6 g/(L h) for glucose and 0.2 g/(L h) for xylose. Further trials were carried out to determine the sensitivity towards common toxic degradation products in lignocellulose hydrolysates: acetate, hydroxymethylfurfural, furfural, formate, levulinic acid and phenolic compounds from hemicellulose fraction. L. lactis was the least tolerant strain towards the inhibitors, whereas L. brevis IGB 1.29 showed the highest tolerance. L. brevis IGB 1.29 exhibited only 10% growth reduction at concentrations of 26.0 g/L acetate, 1.2 g/L furfural, 5.0 g/L formate, 6.6 g/L hydroxymethylfurfural, 9.2 g/L levulinic acid or 2.2 g/L phenolic compounds. This study describes a new strain L. brevis IGB 1.29, that enables efficient lactic acid production with a lignocellulose-derived C5- and C6-sugar fraction.
Introduction Lactic acid is a compound with a broad and promising field of application. On the one hand, it can be used as building block for polymerization to polylactic acid (PLA). On the other hand, it can be applied as platform chemical for many further products, for example acrylic acid, propylene glycol and glycerol [1–3]. Especially the increasing demand for * Susanne Zibek [email protected] 1
Institute of Interfacial Process Engineering and Plasma Technology, University Stuttgart, Nobelstraße 12, 70569 Stuttgart, Germany
2
Present Address: Institute for Bio‑ and Geosciences: Plant Sciences, Forschungszentrum Jülich, Jülich, Germany
3
Present Address: Sartorius Stedim Cellca GmbH, Ulm, Germany
4
Industrial Biotechnology, Fraunhofer Institute of Interfacial and Bioprocess Engineering, Stuttgart, Germany
5
Present Address: Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany
ethyl lactate and PLA lead to a higher production of lactic acid [4]. Currently, lactic acid is produced by chemical as well as by fermentative
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