Marker-assisted identification of novel genetic lines for salinity tolerance and their categorization for utilization in
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ORIGINAL PAPER
Marker‑assisted identification of novel genetic lines for salinity tolerance and their categorization for utilization in development of hybrid rice (Oryza sativa L.) P. Raghavendra1 · B. M Dushyantha Kumar1 · P. Shashikumara2 · H. M Sachin Kumar1 · S. L. Krishnamurthy3 · Shailaja Hittalmani4 Received: 12 July 2020 / Accepted: 24 October 2020 © Akadémiai Kiadó Zrt. 2020
Abstract Identification of new sources of salinity-tolerant genotypes is prerequisite for rice breeding programs in different saline ecosystems. In the present experiment, we characterized 177 landraces from the Western Ghats of Karnataka under natural saline field conditions for two years using morpho-physiological and grain quality parameters. Significant variation was present among landraces for seedling stage and reproductive stage salinity tolerance. The nutrient composition analysis of grain revealed an increase in average grain protein and carbohydrate content under saline conditions. Evaluation of twenty-two SSR markers associated with the Saltol region validated RM140, RM287 and RM3412 as best markers to classify landraces for saline tolerance. Polymorphism Information Content and genetic diversity indices showed that the markers RM10748 and RM10864 were highly useful for distinguishing landraces. Further, to benefit the exploitation of heterosis, eleven maintainers were identified among tolerant landraces and these genotypes could be further developed into male sterile lines for production of salinity-tolerant rice hybrids. Comparison with ‘Pokkali’ for morpho-physiological traits along with molecular confirmation showed that the landraces ‘Doddabaikalu,’ Kalaadikonda,’ Gajagunda’ and ‘Anekombina batha’ were superior donors carrying genomic regions for salinity tolerance. Keywords Rice · Salinity tolerance · Landraces · Saltol
Introduction
Communicated by M. Taylor. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42976-020-00104-2) contains supplementary material, which is available to authorized users.
Rice (Oryza sativa L.) is one of the most important grains in Asian countries and it is consumed by two thirds of the world population as the staple food. World population is increasing rapidly, and there will be a need to produce 70% more rice than we are producing today by 2050, despite the numerous biotic and abiotic constraints (Reddy et al. 2017). Salinity is the second most widespread abiotic constraint
* P. Shashikumara [email protected]
Shailaja Hittalmani [email protected]
P. Raghavendra [email protected]
1
B. M Dushyantha Kumar [email protected]
Department of Genetics and Plant Breeding, UAHS, Shivamogga 577204, India
2
H. M Sachin Kumar [email protected]
ICAR-Indian Grassland and Fodder Research Institute, Jhansi 284003, India
3
ICAR-Central Soil Salinity Research Institute, Karnal, India
4
Department of Genetics and Plant Breeding, UAS, Banglore 560065, India
S. L. Krishnamurthy krishnag
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