Comparison of Polymerase Chain Reaction (PCR), Microbiological and Histopathological Observations in the Diagnosis of En
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ORIGINAL ARTICLE
Comparison of Polymerase Chain Reaction (PCR), Microbiological and Histopathological Observations in the Diagnosis of Endometrial Tuberculosis S. Meenu1 · Sudha Ramalingam1 · Thiagarajan Sairam1,4 · Arati Appinabhavi2 · Seetha Panicker2 · Seema Oommen3 · Ramalingam Sankaran1 Received: 20 January 2020 / Accepted: 28 July 2020 © Federation of Obstetric & Gynecological Societies of India 2020
Abstract Background Female genital tuberculosis often faces diagnostic challenges due to the asymptomatic nature of the disease. Our study aims at comparing the microbiological and histopathological results with PCR in diagnosing genital tuberculosis in endometrial curettage specimens. Methods Around 139 patients with diverse gynaecological complaints were recruited for the study, and endometrial curettage specimens were collected. The specimens were subjected to microbiological culture and staining, histopathological examination and PCR to look for the presence of M. tuberculosis. Statistical analyses of the PCR results include calculating sensitivity, specificity, positive and negative prediction values and positive and negative likelihood ratios. Results PCR yielded a detection rate of 41.7% (58/139) when compared to the microbiology (2.15%) and histopathology results (1.43%). PCR with hsp65 and cfp10, in combination, detected 20% of the cases. Statistical analyses were suggestive that PCR with hsp65 showed a higher sensitivity and specificity of 50% and 92.59% respectively. Conclusion The results obtained in this study suggest that for a definitive diagnosis, combinations of the results from various diagnostics techniques can only be considered. Keywords PCR · Female genital tuberculosis · Culture · Histopathology
Introduction S. Meenu, Sudha Ramalingam and Thiagarajan Sairam have contributed equally to this work. S. Meenu is a Postdoctoral fellow, PSG Center for Molecular Medicine & Therapeutics, PSG Institute of Medical Sciences & Research, Coimbatore, Tamil Nadu, India. Sudha Ramalingam is the Director—Research & Innovation, Professor, PSG Center for Molecular Medicine & Therapeutics, PSG Institute of Medical Sciences & Research, Coimbatore, Tamil Nadu, India. Arati Appinabhavi is an Associate Professor, Department of Obstetrics and Gynecology, PSG Hospitals, Coimbatore, Tamil Nadu, India. Seetha Panicker is a Professor, Department of Obstetrics and Gynecology, PSG Hospitals, Coimbatore, Tamil Nadu, India. Ramalingam Sankaran is the Dean, PSG Institute of Medical Sciences & Research, Coimbatore, Tamil Nadu, India. * Ramalingam Sankaran [email protected] 1
PSG Center for Molecular Medicine and Therapeutics, PSG Institute of Medical Sciences and Research (Affiliated to the Tamil Nadu Dr MGR Medical University), Coimbatore, Tamil Nadu 641 004, India
Female genital tuberculosis continues to be an important cause for infertility, especially in developing countries like India accounting for about 7–15% [1]. This is a form of secondary tuberculosis after a primary encounter of tuberculosis, either
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