Comparison of the regeneration induced by acellular nerve allografts processed with or without chondroitinase in a rat m
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Comparison of the regeneration induced by acellular nerve allografts processed with or without chondroitinase in a rat model Jin-Hyung Im . Joo-Yup Lee . Saerom Lee . Myung-Gyu Lee . Yang-Guk Chung . Ki-Won Kim
Received: 12 December 2018 / Accepted: 19 April 2019 / Published online: 27 April 2019 Ó Springer Nature B.V. 2019
Abstract There have been various studies about the acellular nerve allograft (ANA) as the alternative of autologous nerve graft in the treatment of peripheral nerve defects. As well as the decellularization process methods of ANA, the various enhancement methods of regeneration of the grafted ANA were investigated. The chondroitin sulfate proteoglycans (CSPGs) inhibit the action of laminin which is important for nerve regeneration in the extracellular matrix of nerve. Chondroitinase ABC (ChABC) has been reported that it enhances the nerve regeneration by degradation of CSPGs. The present study compared the regeneration of ANA between the processed without ChABC group and the processed with ChABC group in a rat sciatic nerve 15 mm gap model. At 12 weeks postoperatively, there was not a significant difference in the histomorphometric analysis. In the functional analysis, there were no significant differences in maximum isometric tetanic force, wet muscle weight of tibialis
J.-H. Im Department of Orthopedic Surgery, Gyeongsang National University Changwon Hospital, Changwon, Korea J.-Y. Lee (&) Y.-G. Chung K.-W. Kim Department of Orthopedic Surgery, The Catholic University of Korea College of Medicine, Seoul, Korea e-mail: [email protected] S. Lee M.-G. Lee Korea Public Tissue Bank, Seoul, Korea
anterior. The processed without ChABC group had better result in ankle contracture angle significantly. In conclusion, there were no significant differences in the regeneration of ANA between the processed without ChABC group and the processed with ChABC group. Keywords Acellular nerve allograft Chondroitin sulfate proteoglycans Chondroitinase Peripheral nerve regeneration Processed nerve allograft Rat model
Introduction Nerve defects may occur due to various events, such as trauma, neuroma resection, and tumor resection, and autologous nerve grafts are the standard treatment. However, this approach has disadvantages, including donor site morbidity (pain, loss of sensation, and neuroma occurrence), limitation of donor site choice, an increase in surgery time, and limitations in graft diameter and length. To overcome these disadvantages and identify a substitute for autologous nerve grafts, much research on the conduit properties of various materials, including living tissues such as veins and muscles, has been performed. Synthetic nerve conduits have been manufactured with FDA approval, and to date, several products have been applied in clinical practice. Nerve allografts have also been studied since 1876 as an alternative to autologous nerve grafts
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(Schmidt 1993). The concept of decellularization was introduced to reduce the immun
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