Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y
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Comprehensive cytogenomic profile of the in vitro neuronal model SH-SY5Y Mohammed Yusuf & Kay Leung & Keith J. Morris & Emanuela V. Volpi
Received: 12 June 2012 / Accepted: 18 November 2012 / Published online: 9 December 2012 # The Author(s) 2012. This article is published with open access at Springerlink.com
Abstract The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and clinical significance. An important feature of the SH-SY5Y cells is their ability to differentiate into a functionally mature neuronal phenotype. This property has conferred them the potential to be used as an in vitro model for studies of neurodegenerative and neurodevelopmental disorders. Here, we present a comprehensive assessment of the SH-SY5Y cytogenomic profile. Our results advocate for molecular cytogenetic data to inform the use of cancer cell lines in research. Keywords SH-SY5Y . Neuroblastoma . Cytogenomic . Neuronal Model . Molecular cytogenetics . Karyotype
Introduction Cancer cell lines are extensively used as models to investigate the genetics and behaviour of specific tumours. More M. Yusuf : K. Leung : K. J. Morris : E. V. Volpi (*) Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, OX3 7BN, Oxford, UK e-mail: [email protected] URL: http://www.ndm.ox.ac.uk/principal-investigators/researcher/ emanuela-volpi M. Yusuf London Centre for Nanotechnology, University College London, WC1E 6BT, London, UK Present Address: K. Leung Centre for Genetics and Genomics, Queens Medical Centre, University of Nottingham, NG7 2UH, Nottingham, UK
generally, they are an important resource for basic research on diverse aspects of cellular biology, differentiation and pathology. The widely studied SH-SY5Y human neuroblastoma cell line provides a classic example of how a cancer cell line can be instrumental for discoveries of broad biological and medical significance. Neuroblastoma is a paediatric malignancy of neuroectodermal origin, characterised by genetic heterogeneity and variable clinical progression. The SH-SY5Y cell line is a third successive sub-clone of the SK-N-SH line, originally established from a bone marrow biopsy of a metastatic neuroblastoma patient [1]. The SK-N-SH parental line comprises at least two morphologically and biochemically distinct phenotypes: neuroblastic (N-type), that led to the subcloning of SH-SY5Y (neuroblast-like), and substrate adherent, non-neuronal form (S-type), that led to the sub-cloning of SH-EP (epithelial-like). Different theories have been postulated with regard to the possible biological phenomenon behind the co-existence of those two different cellular phenotypes. Trans-differentiation or the ability of neuroblastoma cells to interconvert bi-directionally, in vitro, from a neuroblast (N) to a non-neuronal (S) form was the initial explanation [2]. Later, “clonal expansion”, or the ability of one of the clones co-existing in the pare
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