Contribution of Lignin Peroxidase, Manganese Peroxidase, and Laccase in Lignite Degradation by Mixed White-Rot Fungi
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ORIGINAL PAPER
Contribution of Lignin Peroxidase, Manganese Peroxidase, and Laccase in Lignite Degradation by Mixed White‑Rot Fungi Kaiyi Shi1,2 · Yi Liu3 · Peng Chen1,2 · Yu Li1,2 Received: 21 April 2020 / Accepted: 5 October 2020 © Springer Nature B.V. 2020
Abstract The catalytic depolymerization of biological enzymes during biological liquefaction of coal is very important. In this study, the effects of medium types, lignite addition, and mixed culture on lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase (Lac) secreted by white-rot fungi are investigated. Fifteen strains of white-rot fungi are used for microbial liquefaction of the Zhaotong lignite in Yunnan. The results show that the activities of LiP, MnP, and Lac are highest in the Czapek-Dox medium (DOX), followed by the Sabouraud Maltose Broth (SMB) medium, and are almost zero in the Potato Dextrose Agar (PDA) medium. In the early stage of fungal growth, the medium contains sufficient nutrients, and lignite addition induces an inhibitory effect on extracellular enzyme secretion, whereas in the late growth stage characterized by nutrient deficiency, it exhibits an enhancing effect. For fungi pair combinations, if only single enzyme activity was considered, the positive synergistic effect of Lac in the mixed culture of s.commune and SL203 would be the highest, with the synergistic coefficient of 233.33. While three enzymes were considered, the integrated effects of ZTW07 and SL203 are the highest, with Lac, MnP, and LiP producing synergistic coefficients of 158.795, 1.217, and 1.006, respectively. It can be seen that the mixed culture can greatly improve the enzyme activity of white rot fungi and is one of the effective methods to improve the liquefaction efficiency. Graphic Abstract
Keywords Lignite · White-rot fungi · Lignin peroxidase · Manganese peroxidase · Laccase · Synergistic coefficient
* Kaiyi Shi [email protected] Extended author information available on the last page of the article
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Statement of Novelty (1) In this paper, the method of fungus mixed culture was applied to the lignite microbial liquefaction, and it was found that the activity of Lac enzyme increased 428 times subsequent to two fungi mixed cultures. (2) To evaluate the activities of Lip, MnP, and Lac, the coefficient of synergistic effect was introduced to determine the best mixed culture combination of enzymolysis fungi.
Introduction Lignite is a low-rank coal with a lignin-like structure [1, 2]. Due to its low heating value, high moisture content, weathering susceptibility, and easy self-ignition, it is unsuitable as coking coal [3, 4], and cannot replace coal as a better fuel. At present, it is mainly employed in power generation, although pollution problems such as smog and the greenhouse effect of carbon dioxide caused many countries to shut such power plants [5, 6]. In addition, lignite is also treated with strong alkalis to extract humic acid, but the associated secondary pollution created remains unsolved [7]. Therefore, the
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