Molecularly imprinted polyaniline for detection of horseradish peroxidase
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RESEARCH PAPER
Molecularly imprinted polyaniline for detection of horseradish peroxidase Pavel S. Pidenko 1 & Sergei A. Pidenko 1 & Yulia S. Skibina 2 & Andrey M. Zacharevich 1 & Daniil D. Drozd 1 & Irina Yu Goryacheva 1 & Natalia A. Burmistrova 1 Received: 24 March 2020 / Revised: 22 April 2020 / Accepted: 28 April 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A new facile and fast approach to the synthesis of polyaniline (PANi) molecularly imprinted polymers (MIPs) based on aniline oxidative chemical polymerization was proposed for protein recognition. For the first time, a surface imprinting strategy was implemented for the synthesis of PANi MIPs on the inner surface of soft glass polycapillaries (PC) with a large (2237) number of individual microcapillaries. Two different PANi layers—(i) PANi film and (ii) protein imprinted PANi nanowires—were synthesized sequentially. Uniform and highly stable PANi film was synthesized by oxidative polymerization at pH< 1. The synthesis of PANi MIPs on the PANi film pre-coated surface improved the reproducibility of PANi MIP formation. PANi MIP nanowires were synthesized at “mild” conditions (pH > 4.5) to preserve the protein template activity. The binding of horseradish peroxidase (HRP) molecules on the PANi MIP selective sites was confirmed by photometry (TMB chromogenic reaction), SEM images, and FTIR spectroscopy. The developed PANi MIPs enable HRP determination with a limit of detection (LOD) as low as 1.00 and 0.07 ng mL−1 on the glass slips and PC, respectively. The PANi MIPs are characterized by high stability; they are reversible and selective to HRP. The proposed approach allows PANi MIPs to be obtained for proteins on different supports and to create new materials for separation and sensing. Keywords Molecular imprinting . Protein . Polyaniline . Horseradish peroxidase . Glass polycapillary
Introduction Fundamental studies of the protein functions in living organisms, as well as the application of protein analysis to solving problems of biology, medicine, proteomics [1], and food analysis [2], are one of the most important fields of science for a long time. It is therefore not surprising that rapid increase in the development of highly efficient methods of protein
Published in the topical collection featuring Female Role Models in Analytical Chemistry. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00216-020-02689-3) contains supplementary material, which is available to authorized users. * Natalia A. Burmistrova [email protected] 1
Institute of Chemistry, Saratov State University, Astrakhanskaya 83, Saratov, Russia 410012
2
SPE LLC Nanostructured Glass Technology, Saratov 410033, Russia
purification, characterization, quantitation, detection [3, 4], and separation techniques [5, 6] has been seen. The molecular recognition which plays an important role in biological systems nowadays became a powerful tool in protein determination. The formation of structures based on natural non
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