Conventional ICSI improves the euploid embryo rate in male reciprocal translocation carriers
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ASSISTED REPRODUCTION TECHNOLOGIES
Conventional ICSI improves the euploid embryo rate in male reciprocal translocation carriers Caixia Lei 1 & Shuo Zhang 1 & Saijuan Zhu 1 & Junping Wu 1 & Min Xiao 1 & Jing Zhou 1 & Jing Fu 1 & Yijuan Sun 1 & Congjian Xu 1,2 & Xiaoxi Sun 1,2,3 Received: 7 July 2020 / Accepted: 10 November 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Purpose To evaluate whether the morphologically normal spermatozoa selected for intracytoplasmic sperm injection (ICSI) under microscope had a higher rate of normal/balanced chromosome contents than that in the whole unselected sperm from reciprocal translocation carriers. Methods Five hundred unselected spermatozoa from each of 40 male translocation carriers were performed with fluorescence in situ hybridization (FISH), to determine the rates of gametes with different meiotic contents of translocated chromosomes. Meanwhile, 3030 biopsied blastocysts from 239 male and 293 female reciprocal translocation carriers were detected with the microarray technique to analyze the rates of embryos with different translocated chromosome contents. Results The D3 embryo rate, blastocyst formation rate, and euploid rate of blastocysts were remarkably higher in male carriers than those in female (p = 0.001, p = 0.004, and p = 0.035, respectively). In addition, the percentages of alternate products, which contained normal/balanced chromosome contents, in embryos from male carriers were markedly higher than those in sperm FISH (p = 2.48 × 10−5 and p = 2.88 × 10−10), while the percentages of adjacent-2 and 3:1 products were lower than those in sperm FISH (p = 0.003 and p = 5.28 × 10−44). Moreover, consistent results were obtained when comparing the rates of products in embryos between male and female carriers. Specifically, the incidence of alternate products in male carriers was higher than those in female carriers (p = 0.022). However, no similar differences were seen between sperm and embryos of female carriers. Conclusion ICSI facilitates the selection of spermatozoa with normal/balanced chromosome contents and improves the D3 embryo rate, blastocyst formation rate, and the euploid embryo rate in male carriers. Keywords Male reciprocal translocation . Sperm FISH . ICSI . Quadrivalent structure . Segregation patterns . Alternate products
Introduction Palermo et al. had first reported the microscopic selection of morphologically normal spermatozoon (magnifications: × 200/400) to be used in intracytoplasmic sperm injection (ICSI) [1]. Typically, introducing the ICSI procedures into Caixia Lei and Shuo Zhang contributed equally to this work. * Xiaoxi Sun [email protected] 1
Shanghai Ji Ai Genetics & IVF Institute, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China
2
Key Laboratory of Female Reproductive Endocrine Related Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China
3
Shanghai, China
the assisted reproduction techniques has marked an important milestone in
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