CRE/LOX-based analysis of cell lineage during root formation and regeneration in Arabidopsis
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CRE/LOX-based analysis of cell lineage during root formation and regeneration in Arabidopsis Ning Zhai1,2 , Lin Xu1& 1
2
National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China
Received: 11 May 2020 / Accepted: 25 June 2020
Abstract
The root system of Arabidopsis thaliana comprises primary, lateral, and adventitious roots. Different types of roots are formed by diverse inductive cues and developmental programs. Here, we adopted the CRE/LOX system to trace cell lineage during the three types of root formation under the control of the promoter of WUSCHEL-RELATED HOMEOBOX5. The results show that the cells forming adventitious roots during de novo root regeneration from detached leaves and lateral roots from the primary root are descendants of the WOX5-expressing root primordium. During the post-embryonic growth of the primary root, some vascular and root cap cells are descendants of the WOX5-expressing stem cell niche in the root apical meristem. Overall, our data suggest that the CRE/LOX system is a useful tool to trace cell lineage in different types of root organogenesis.
Keywords Root regeneration, Adventitious root, Lateral root, CRE/LOX, WOX5, Arabidopsis thaliana
DEAR EDITOR, During postembryonic development, Arabidopsis thaliana can form different types of roots. The pericyclederived acropetal lateral roots (hereafter referred to as lateral roots) are formed from an existing root (e.g. the primary root) and the adventitious roots are mainly formed from non-root organs. The formation of both lateral and adventitious roots requires the establishment of root founder cells and then the division of root founder cells initiates the root primordium. During adventitious rooting from detached leaves (known as de novo root regeneration, Fig. 1A), wound-induced auxin accumulation promotes cell fate transition from a regeneration-competent cell (i.e. procambium and some vascular parenchyma cells) into an adventitious root founder cell, and then the division of the adventitious
& Correspondence: [email protected] (L. Xu)
root founder cell initiates the adventitious root primordium (Liu et al. 2014; Hu and Xu 2016; Xu 2018). During lateral rooting from the primary root, root capderived auxin flux activates several pairs of xylem-pole pericycle cells to become the lateral root founder cells, and then asymmetric division of those lateral root founder cells initiates the lateral root primordium (Xuan et al. 2015, 2016; Du and Scheres 2018; Motte et al. 2019; Banda et al. 2019). Here, we adopted the CRE/LOX system (Metzger et al. 1995; Brocard et al. 1998; Smetana et al. 2019) to trace the cell lineage during adventitious, lateral and primary root formation under the control of the WUSCHELRELATED HOMEOBOX5 (WOX5) promoter (WOX
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