Creation of Certified Reference Material of Ascorbic Acid Composition
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PHYSICOCHEMICAL MEASUREMENTS CREATION OF CERTIFIED REFERENCE MATERIAL OF ASCORBIC ACID COMPOSITION M. P. Krasheninina,1 O. S. Shokhina,1 A. S. Sergeeva,1 T. N. Tabatchikova,1 V. B. Baranovskaya,2 and Yu. A. Karpov2
UDC 006.9:53.089.68:543.24
The article presents the application of the “one hundred minus sum of impurities” approach in the development of a reference material of ascorbic acid composition using state primary and secondary measurement standards. High-performance liquid chromatography (HPLC) with a diode-array detector is used for the quantitative determination of impurities of similar structure and with the highest content compared to other impurities. Traceability of certified values was provided using the calibration method of the State Primary Standard of units of mass (molar) fraction and mass (molar) concentration of organic components in liquid and solid substances and materials based on liquid and gas chromatography-mass spectrometry with isotope dilution and gravimetry GET 208-2019. The paper presents the use of the reference material of ascorbic acid composition to test the qualifications of laboratories. Satisfactory results of the participants confirmed the applicability of the reference material for various measurement methods. Keywords: measurement standards, certified reference materials, gas chromatography, liquid chromatography, traceability of measurement results, CRM of ascorbic acid composition.
Introduction. Ascorbic acid is one of the main substances in the human diet, necessary for normal life [1]. Humans are not able to synthesize ascorbic acid on their own, so it must be ingested with food and pharmaceuticals. Ascorbic acid is actively used in the pharmaceutical industry as a pharmaceutical substance, such as in the manufacture of medicines for various purposes, including vitamin preparations [2]. Thus, it is obvious that the content of ascorbic acid must be controlled by chemical analysis methods, both in the process of creating pharmaceuticals based on it, and in the analysis of food products. Quantitative determination of ascorbic acid is carried out using various methods: titrimetry [3]; spectrophotometry [3] (GOST 31717-2012, “Juices and juice products. Identification. Determination of ascorbic acid by the enzymatic method”), high performance liquid chromatography (HPLC) [4–6] (GOST 31643-2012, “Juice products. Determination of ascorbic acid by high performance liquid chromatography”), capillary electrophoresis (GOST R 53193-2008, “Alcoholic and non-alcoholic beverages. Determination of caffeine, ascorbic acid and its salts, preservatives, and sweeteners by capillary electrophoresis”), potentiometry (GOST 24556-89, “Products of processing fruits and vegetables. Methods for determining vitamin C”) and others. For metrological support and accuracy control of quantitative determination of ascorbic acid by all of the above methods, a certified reference material (CRM) of pure ascorbic acid composition is required. Currently, in accordance with the recommendations of the Inter
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