CREBZF expression and hormonal regulation in the mouse uterus
- PDF / 910,340 Bytes
- 9 Pages / 595.28 x 793.7 pts Page_size
- 96 Downloads / 185 Views
RESEARCH
Open Access
CREBZF expression and hormonal regulation in the mouse uterus Pengfei Lin1,2, Fenglei Chen1,2, Nan Wang1,2, Xiangguo Wang1,2, Xiao Li1,2, Jinhua Zhou1,2, Yaping Jin1,2 and Aihua Wang2*
Abstract Background: CREBZF is a member of the mammalian ATF/CREB family of the basic region-leucine zipper (bZIP) transcription factors. Two isoforms of CREBZF have been identified from the alternative usage of initiation codons, SMILE (long isoform of CREBZF) and Zhangfei (short isoform of CREBZF). Until recently, the physiological function of CREBZF in mammalian reproductions has not been reported. Methods: Multiple techniques were performed to investigate the spatiotemporal expression and hormonal regulation of the CREBZF gene in the mouse uterus and its role in embryo implantation. Results: Zhangfei was not detected in the mouse uterus. SMILE immunostaining was mainly expressed in the uterine luminal and glandular epithelium, and the expression levels of both SMILE mRNA and protein gradually decreased from days 1–3 of pregnancy, peaked on day 4, and then declined again on day 6. On day 5 of pregnancy, SMILE protein expression was detected only in the luminal epithelium at implantation sites compared with the expression at inter-implantation sites. SMILE protein was not detected in decidual cells from days 6–8 of pregnancy or artificial decidualisation. Furthermore, SMILE protein was not detected in the mouse uterus on days 3–6 of pseudopregnancy, and SMILE expression was also induced in the delayed-implantation uterus, indicating that the presence of an active blastocyst was required for SMILE expression at the implantation site. Oestrogen significantly stimulated SMILE expression in the ovariectomised mouse uterus. In addition, in cycling mice, high levels of SMILE protein and mRNA expression were also observed in proestrus and oestrus uteri. Conclusions: Taken together, these results suggested that SMILE expression was closely related to mouse implantation and up-regulated by oestrogen. Keywords: Embryo implantation, Mouse, Uterus, CERBZF, SMILE
Background Successful implantation is dependent on the intricate genetic and molecular signalling dialogue between the receptive uterus and active embryo [1]. A better understanding of the molecular events underlying the regulation of embryo implantation may improve the ability to treat infertility. To date, although many molecular modulators have been identified during the implantation period, the precise molecular mechanism underlying embryo implantation is still unknown. CREBZF is as a member of the CREB (cAMP-responseelement-binding protein)/ATF (activating transcription * Correspondence: [email protected] 2 College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China Full list of author information is available at the end of the article
factor) family of basic region-leucine zipper (b-ZIP) transcription factors [2]. Previously studies have found that CREBZF gene expression produces two isoforms, CREBZF-L (long isoform of C
Data Loading...
