Expression of Eya1 in mouse taste buds
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Expression of Eya1 in mouse taste buds Makoto Ohmoto1 · Satsuki Kitamoto2 · Junji Hirota1,2 Received: 7 August 2020 / Accepted: 24 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Taste substances are detected by taste receptor cells in the taste buds in the oral epithelium. Individual taste receptor cells contribute to evoking one of the five taste qualities: sweet, umami, bitter, sour, and salty (sodium). They are continuously replaced every few weeks by new ones generated from local epithelial stem cells. A POU transcription factor, Pou2f3 (also known as Skn-1a), regulates the generation and differentiation of sweet, umami, and bitter cells. However, the molecular mechanisms underlying terminal differentiation into these Pou2f3-dependent taste receptor cells remain unknown. To identify the candidate molecules that regulate the differentiation of these taste receptor cells, we searched for taste receptor typespecific transcription factors using RNA-sequence data of sweet and bitter cells. No transcription factor gene showing higher expression in sweet cells than in bitter cells was found. Eyes absent 1 (Eya1) was identified as the only transcription factor gene showing higher expression in bitter cells than in sweet cells. In situ hybridization revealed that Eya1 was predominantly expressed in bitter cells and also in the putative immature/differentiating taste bud cells in circumvallate and fungiform papillae and soft palate. Eya1 is a candidate molecule that regulates the generation and differentiation of bitter cells. Keywords Taste · Taste receptor cell · Bitter · Eya1 · Differentiation
Introduction Taste substances in foods are detected by specialized sensory cells called the taste receptor cells in the taste buds that are localized in the circumvallate and foliate papillae in the posterior tongue, fungiform papillae in the anterior tongue, and soft palate. Individual taste cells contribute to evoking one of the five taste qualities: sweet, umami, bitter, salty (sodium), and sour. Of these tastes, sweet, umami, and bitter tastes are detected by G protein-coupled receptors: Tas1r2/ Tas1r3 for sweet, Tas1r1/Tas1r3 for umami, and Tas2rs for Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00441-020-03311-9) contains supplementary material, which is available to authorized users. * Makoto Ohmoto [email protected] * Junji Hirota [email protected] 1
Center for Biological Resources and Informatics, Tokyo Institute of Technology, Yokohama 226‑8501, Japan
Department of Life Science and Technology, Graduate School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226‑8501, Japan
2
bitter (Chandrashekar et al. 2006; Yarmolinsky et al. 2009). Humans and mice have 35 and 25 Tas2rs in their genome, respectively, and each bitter cell expresses multiple Tas2rs (Adler et al. 2000; Mueller et al. 2005). Sweet, umami, and bitter cells commonly express the signal transduction
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