Culture condition optimization and FT-IR analysis of Polygonum multiflorum Thunb. adventitious root cultures grown in an
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ORIGINAL ARTICLE
Culture condition optimization and FT‑IR analysis of Polygonum multiflorum Thunb. adventitious root cultures grown in an air‑lift bioreactor system Thanh‑Tam Ho1,2 · Kim‑Cuong Le3,4 · Suk‑Weon Kim5 · So‑Young Park3 Received: 22 July 2020 / Accepted: 24 October 2020 © Springer Nature B.V. 2020
Abstract Bioreactor cultures have been used for biomass production and bioactive compounds accumulation in adventitious root cultures of medicinal plants. In this study, we evaluated the effects of different auxin types [indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)] and concentrations (0.5, 1.0, 2.0 and 4.0 mg·L−1), Murashige and Skoog (MS) medium salt strength (0.25, 0.5, 0.75, 1.0, 1.5 and 2X), and sucrose concentrations (0, 1.5, 3, 5, 7 and 10%) on Polygonum multiflorum Thunb. adventitious root cultures in a 3-L balloon-type bubble bioreactor (BTBB). IBA (1, 2, and 4 mg·L−1) was more effective than NAA in promoting root growth. Additionally, low MS salt strength (0.25 and 0.5X MS) increased the accumulation of total phenolics and flavonoids but reduced biomass accumulation. Four weeks of culture in full-strength MS medium supplemented with 2 mg·L−1 IBA and 5% sucrose resulted in the highest root biomass [98.46 g·L−1 fresh weight (FW); 13.46 g·L−1 dry weight (DW)] and bioactive compounds accumulation (total phenolics compounds, 53.08 mg·g−1 DW; total flavonoids, 25.10 mg·g−1 DW). To determine whether metabolic fingerprinting of whole-cell extracts could be used to compare metabolic equivalence of P. multiflorum root samples, we treated adventitious roots with different culture conditions, and analyzed the treated adventitious roots and natural roots by Fourier transform infrared (FT-IR) spectroscopy. The results showed that the metabolic pattern of adventitious root samples was similar under different culture conditions; however, these samples could be discriminated from each other in pilot-scale bioreactors. Overall, our study provides useful information for industrial-scale cultivation of P. multiflorum adventitious roots. Key message The optimum condition for bioreactor culture of Polygonum multiflorum is full-strength MS medium supplemented with 2 mg·L-1 IBA and 5% sucrose after 4 weeks of culture. FT-IR analysis can rapidly discriminate among root samples based on total metabolite equivalence. Keywords Adventitious root · Bioactive compounds · Bioreactor culture · FT-IR · Root biomass Communicated by Wagner Campos Otoni. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11240-020-01961-9) contains supplementary material, which is available to authorized users. * So‑Young Park [email protected] 1
Institute for Global Health Innovations, Duy Tan University, Da Nang 550000, Vietnam
2
Faculty of Pharmacy, Duy Tan University, Da Nang 550000, Vietnam
3
Department of Horticulture, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea
4
Department of Forest
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