De novo assembly of transcriptome dataset from leaves of Dryobalanops aromatica (Syn. Dryobalanops sumatrensis ) seedlin
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DATA NOTE
De novo assembly of transcriptome dataset from leaves of Dryobalanops aromatica (Syn. Dryobalanops sumatrensis) seedlings grown in two contrasting potting media Iskandar Zulkarnaen Siregar1* , Fifi Gus Dwiyanti1, Ulfah Juniarti Siregar1 and Deden Derajat Matra2
Abstract Objectives: Efforts to restore tropical peat swamp forests in Indonesia face huge challenges of potential failures due to socio-economic factors and ecological dynamics attributed to lack of knowledge on the adaptive mechanisms of potential tree species such as Kapur (Dryobalanops aromatica C.F.Gaertn Syn. Dryobalanops sumatrensis J.F. Gmelin A.J.G.H Kostermans). This species is a multi-purpose tree that, commonly grows in mineral soils, but also in peat swamp as previously reported, which raised a fundamental question regarding the molecular mechanism of this adaptation. Therefore, a dataset was created aiming to detect candidates of adaptive genes in D. aromatica seedlings, cultivated in two contrasting potting media, namely mineral soil and peat media, based on RNA Sequencing Transcriptome Analysis. Data description: The RNA transcriptome data of D. aromatica’s seedlings derived from young leaves of three one-year-old seedlings, raised in each dry mineral soil media and peat media, were generated by using Illumina HiSeq 4000 platform in NovogenAIT, Singapore. The acquired data, as the first transcriptome dataset for D. aromatica, is of a great importance in understanding molecular mechanism and responses of the involved genes of D. aromatica to the contrasting, growing potting media conditions that could also be useful to generate molecular markers. Keywords: Adaptive genetic variation, Dryobalanops aromatica, Dryonalanops sumatrensis, Peat swamp, Transcriptome Objective The past genetic research on Dryobalanops aromatica focused on pattern of genetic variation and population structure in North-eastern Borneo, Sumatera, and the Malay Peninsula using nuclear microsatellite markers [1]. The investigated ecosystem types for all populations were from mineral soil forest types, in which D. aromatica could be found abundantly on deep, humid, yellow, *Correspondence: [email protected] 1 Department of Silviculture, Faculty of Forestry and Environment, IPB University (Bogor Agricultural University), Bogor, Indonesia Full list of author information is available at the end of the article
sandy soils with a propensity for ridges [2]. However, it was recently discovered that this species also grows in peat swamp forest, as found in Singkil Wildlife Reserve (Suaka Margasatwa Singkil), Aceh, Sumatera. According to this finding, the former investigation was then concentrated on how to understand life-history characteristics such as comparing shoot cuttings ability of D. aromatica in peat and coco peat media [3]. In addition, due to lack of in-depth investigation of adaptive genetic variation of this species grown in mineral soil and peat media, an experiment was carried out through RNA sequencing (RNA-Seq) tra
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