Deconvoluted plasma DSC curves on patients with psoriasis
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Deconvoluted plasma DSC curves on patients with psoriasis Dénes Lőrinczy1 · Mehdi Moezzi2 · Andrea Ferencz3 Received: 6 August 2019 / Accepted: 11 February 2020 © The Author(s) 2020
Abstract Psoriasis is an inflammatory disease that changes plasma composition, and it is detectable by differential scanning calorimetry (DSC). Besides the general change in plasma, the aim of the study was to demonstrate which components are changing and how the anti-psoriatic drug treatment affects back all this. Retrospectively, blood plasma DSC data were analyzed from patients, who have different severities of symptoms and who received steroids (n = 10), or retinoids (n = 10), or biological drug treatment (n = 10). Complex curves were deconvoluted in several individual transitions (T m1–Tm5), modeling each individual transition. In the examined psoriasis stages, the thermodynamic parameters excess heat capacity and enthalpy of the transitions in proportion corresponded to the targeted treatment and the degree of disease severity, as well as the numbers of transitions were determined from the calorimetric profiles. In conclusion, deconvoluted plasma DSC profiles showed similarities but exhibited marked differences in the thermal denaturation on different treated psoriasis stages. This examination has shown that drug therapy affects the composition of plasma proteins, which should be always considered for the evaluation of DSC results in similar studies. Keywords Psoriasis · Blood plasma · DSC · Curve · Deconvolution · Cytostatic therapy · Retinoid therapy · Biological therapy
Introduction Psoriasis is a lifelong skin disorder, which has different appearances and shapes (plaque, nail, scalp, guttate, inverse, pustular, and erythrodermic psoriasis), and in rare cases it can affect the joints (psoriatic arthritis). Symptoms are frequently categorized into two groups: mild or moderate to severe psoriasis. The classification is depending on the
* Andrea Ferencz [email protected] Dénes Lőrinczy [email protected] Mehdi Moezzi [email protected] 1
Institute of Biophysics, Medical School, University of Pécs, Pecs, Szigeti Str. 12, Hungary
2
Department of Dermatology, Venereology and Oncodermatology, Medical School, University of Pécs, Pecs, Akácfa Str. 1, Hungary
3
Department of Surgical Research and Techniques, Faculty of Medicine, Semmelweis University, Budapest, Nagyvárad Sqr. 4, 1089, Hungary
clinical severity of the lesions, the percentage of affected body surface area, and patient quality of life. Clinical diagnosis is usually based on full skin physical examination by a dermatologist, and rarely skin biopsy is useful to determine the exact type of psoriasis. In the case of joint involvement, radiological examinations (X-ray, ultrasound, and MRI) should be necessary to confirm the diagnosis. At present, specific blood tests and/or radiographic findings often not available that reliably confirmed the diagnosis [1–4]. More and more things are known about the pathogenesis of the process, and parallel to thi
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