Decreased CD4+CD152+ T cell subset and its correlation with the level of antithyroid antibodies in children with chronic
- PDF / 383,872 Bytes
- 4 Pages / 597.968 x 844.582 pts Page_size
- 104 Downloads / 162 Views
eUroPeAn JoUrnAL of MediCAL reSeArCh
eur J Med res (2010) 15(Suppl. ii): 72-75
november 4, 2010 © i. holzapfel Publishers 2010
deCreASed Cd4+Cd152+ t CeLL SUBSet And itS CorreLAtion With the LeVeL of Antithyroid AntiBodieS in ChiLdren With ChroniC AUtoiMMUne thyroiditiS A. M. Kucharska1, e. Gorska 2, M. Wasik 2, B. Pyrzak1 1department
of Pediatrics and endocrinology and 2 department of Laboratory diagnostics and Clinical immunology of the developmental Age, Warsaw Medical University, Warsaw, Poland
Abstract Objective: Cytotoxic t lymphocyte antigen-4 (CtLA4) is one of the basic antigens involved in immune responses regulation associated with autoimmune thyroid diseases. the aim of the study was to evaluate whether the surface expression of CtLA-4(Cd152) on t cells is correlated with laboratory autoimmune markers in children with hashimoto’s disease. Material and methods: Blood samples were obtained from 45 children with hashimoto’s thyroiditis of the mean age 14.8 ±2.35 years, and from 55 healthy agematched children, free of allergic, immune and hematological disorders, and with a normal thyroid function. the anti-thyroid antibodies were measured with Microparticle enzyme immunoassay (AxSyM Antitg, AxSyM Anti-tPo). the t cell phenotype was evaluated flow cytometery, with the use of monoclonal antibodies combination: Cd4- fitC/ Cd28 -PC5/ Cd152 -Pe and Cd8 -fitC/ Cd28 -PC5/ Cd152 Pe Results: the percentage of t cells with Cd152 expression was significantly decreased in children with hashimoto's thyroiditis compared with healthy controls (P12 iU/ml. Cell preparation before cytometric analysis was described previously [10]. in brief, heparinized blood samples from ht children and healthy controls were diluted in saline three times, and centrifuged for 30 min by 400 x g on histopaque 1077-1 density gradient from SiGMA diagnostics (St. Louis, Mo, USA). the isolated peripheral blood mononuclear cells (PBMC) were incubated with monoclonal antibodies for 30 min at 25°C in darkness. Analysis was performed with the use of monoclonal antibody combination: Cd4- fitC/Cd28 -PC5/Cd152 -Pe and Cd8 -fitC/Cd28 -PC5/Cd152 -Pe obtained from immunotech Beckman Coulter Company (Beckman Coulter Company, Paris nord, france). After incubation samples were fixed and lysed by the reagent set Uti-Lyse (dako Cytomation, Gdynia, Poland). the t cell phenotype was evaluated using the flow cytometer Beckman Coulter ePiCS XL 4C (ePiCS XL/XLMCL, version 2.0, Beckman Coulter Company, Paris nord, france). results were statistically analyzed with a t-test, Mann-Whitney U test, and Spearman’s test for correlations, as required.
reSULtS in tricolor cytometry, a significantly decreased percentage of t cells with Cd152 expression was found in the children with ht compared with healthy controls: 2.6 ±1.8 and 4.5 ±1.5%, respectively (P
Data Loading...
