Design of Localized Surface Plasmon Resonance (LSPR) Biosensor for Immunodiagnostic of E. coli O157:H7 Using Gold Nanopa
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Design of Localized Surface Plasmon Resonance (LSPR) Biosensor for Immunodiagnostic of E. coli O157:H7 Using Gold Nanoparticles Conjugated to the Chicken Antibody Fatemeh Yaghubi 1 & Mehdi Zeinoddini 1
&
Ali Reza Saeedinia 1 & Azadeh Azizi 1 & Afshin Samimi Nemati 1
Received: 24 December 2019 / Accepted: 22 March 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract E. coli O157:H7 is one of the most important pathogens in food-borne diseases and is the main cause of the pseudo pandemic development of hemorrhagic colitis and hemolytic uremic syndrome. Also E. coli O157:H7 is the most common serotype of Shiga-toxin-producing E. coli. Traditional methods for detecting E. coli O157:H7 are expensive, time-consuming, and less sensitive. A method with high sensitivity and high-resolution optical detection is utilizes the LSPR property of spherical gold nanoparticles (GNP). In this work, we constructed a novel nano-bio probe to detect E. coli O157:H7 by synthesizing citrate gold nanoparticle conjugated (non-covalent bond) with specific chicken anti-E. coli O157:H7 antibody (IgY) by changing the pH of the nanoparticles’ environment. UV-visible and DLS methods were used to confirm the bonding between the antibody and nanoparticles and the LSPR sensitivity of the nano-bio probe was evaluated by ELISA method. We could optically detect this bacterium in less than 2 h by measuring the LSPR band λ max shifts of GNPs. The sensitivity of this novel biosensor was determined by about 10 CFU/ml, using the LSPR property of spherical gold nanoparticles. So that, the LSPR λ max red shifted from 530 to 543 nm in presence of 10 CFU bacterium. In conclusion, this nano biosensor can be used to detect this important pathogen among the clinical specimens. Keywords E. coli O157:H7 . Chicken antibody . Gold nanoparticles . LSPR . Nano-bio probe
Introduction Escherichia coli O157:H7 is the most important strain of E. coli that is one of the Shiga toxin-producing types that produces Shiga like toxin or verotoxin. This toxin can infect humans and cause life-threatening, typically foodborne diseases through the consumption of contaminated and raw foods, including raw milk and undercooked ground beef [1–3]. Infection with this type of pathogenic bacteria may lead to hemorrhagic diarrhea and kidney failure. This strain has been reported to cause death in children below 5 years of age, elderly patients, and patients with immunodeficiency [4–6]. The presence of this bacterium in water and food has been accepted as an indicator of fecal contamination and the possible presence of dominant pathogens. In conclude this
* Mehdi Zeinoddini [email protected] 1
Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Tehran, Iran
serotype is the major cause of hemorrhagic colitis, hemolytic uremic syndrome, and acute renal failure in children, and causes numerous deaths each year [7, 8]. Infections caused by E. coli are common in different countries of the world. During the outbreaks in the
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