Surface plasmon resonance biosensor using hydrogel-AuNP supramolecular spheres for determination of prostate cancer-deri
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ORIGINAL PAPER
Surface plasmon resonance biosensor using hydrogel-AuNP supramolecular spheres for determination of prostate cancer-derived exosomes Wenqin Chen 1 & Jia Li 1 & Xiaotong Wei 1 & Yunpeng Fan 1 & Husun Qian 1 & Siqiao Li 2 & Yu Xiang 3 & Shijia Ding 1,2 Received: 13 July 2020 / Accepted: 25 September 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Based on the hydrogel-AuNP supramolecular sphere (H-Au), a label-free and real-time surface plasmon resonance imaging biosensor has been developed for highly sensitive and specific determination of prostate cancer cell-derived exosomes. After integrating the signal amplification effect of the mass cumulative hydrogel and the LSPR effect of AuNPs with high specific aptamer, the SPRi biosensor for exosome detection exhibited a wide linear range from 1.00 × 105 to 1.00 × 107 particles/mL with a limit of detection of 1.00 × 105 particles/mL. Most importantly, with a strong correlation between the SPRi signal and the t-PSA value measured by the clinical chemiluminescence immunoassay, this biosensor displayed excellent practicability for human serum analysis, which exhibits great potential applications in disease diagnosis and bioanalysis. Keywords Exosomes . Surface plasmon resonance imaging (SPRi) . Hydrogel-gold nanoparticles (hydrogel-AuNPs) . Tumor diagnosis . Prostate-specific antigen (PSA)
Introduction According to the Global Burden of Disease (GBD), there are more than one-fifth of new prostate cancer cases occurred in the USA in 2017, the increasing incidence rate makes it a higher prevalence disease than lung cancer [1, 2]. In addition, prostate cancer can not only cause complications such as acute seminal vesiculitis but also easily lead to bone metastasis, fracture, Wenqin Chen and Jia Li contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04573-4) contains supplementary material, which is available to authorized users. * Shijia Ding [email protected] 1
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
2
Chongqing Engineering Research Center for Criminal Investigation Technology, Chongqing Medical University, Chongqing 400016, China
3
The Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing 40016, China
paraplegia, and even life-threatening [3]. Nowadays, the detection of serum prostate-specific antigen (PSA) is widely used in clinics, which is principally based on chemiluminescence immunoassay to test the content of PSA and related factors in patient serum, providing references for subsequent diagnosis [4]. In spite of this, the deficiencies of low specificity, sensitivity, and high false positive make the method full of controversy. What’s worse, the detection threshold of PSA is also questioned [5]. Under these circumstances, there is no doubt that the introduction o
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