Detection of Inosine Monophosphate (IMP) in Meat Using Double-Enzyme Sensor
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Detection of Inosine Monophosphate (IMP) in Meat Using Double-Enzyme Sensor Guangxian Wang 1 & Jianfei Sun 1 & Yao Yao 1 & Xingshuang An 1 & Hui Zhang 1 & Guanglei Chu 1 & Shui Jiang 2 & Yemin Guo 1 & Xia Sun 1 & Yuan Liu 2 Received: 19 July 2019 / Accepted: 22 September 2019 # Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract Inosine monophosphate (IMP) was considered to be an important component of meat flavor and an important indicator for evaluating the quality of meat products. This paper developed a novel dual-enzyme biosensor for the quantitative detection of inosinic acid to assess meat quality. Using the conductivity of MXene materials and the ability of Au@Pt nanoflowers to catalyze the oxidation of H2O2, a dual-enzyme biosensor was assembled and prepared for sensitive and rapid detection of IMP. The MXene-Ti3C2Tx material had 2D nanostructure similar to that of graphene, as well as metal conductivity and good biocompatibility. MXene was used as a carrier for 5′-nucleotidase and xanthine oxidase, with good biological environment and stable microenvironment. Bimetallic nanoflowers with a core-shell structure had better ability to catalyze H2O2 than the single metal. The double-enzyme hydrolyzed IMP to produce the H2O2. The Au@Pt nanoflowers of the sensor can catalyze the decomposition of H2O2, which causes electron transfer to produce current change. Therefore, the content of IMP is indirectly obtained by monitoring the current change. The results showed that the linear range of the double-enzyme biosensor was 0.04~17 g L−1, the correlation coefficient was 0.9964, and the detection limit was 2.73 ng mL−1. The biosensor had great reproducibility and stability. Compared with high-performance liquid chromatography, biosensors could quickly and accurately detect the content of inosine monophosphate in meat, and provided a better method to detect the quality index of meat products. Keywords Transition metal carbide . Au@Pt nanoflowers . Enzyme biosensor . Inosine monophosphate
Highlights 1. Developed a new dual-enzyme biosensing system for the determination of IMP. 2. Design and synthesis of a novel Mxene(Ti3C2Tx)-Pt@Au nanoflower composite film with conductivity and biocompatibility. 3. The results showed that the enzyme biosensor had a good linear relationship in the range of from 0.04 to 17 g L−1, the correlation coefficient was 0.9964, and the detection limit was 2.73 ng mL−1. 4. A novel enzyme biosensor was developed to assess meat quality and freshness. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12161-019-01652-y) contains supplementary material, which is available to authorized users. * Yemin Guo [email protected] Guangxian Wang [email protected]
1
School of Agricultural Engineering and Food Science, Shandong University of Technology, No. 12 Zhangzhou Road, Zibo 255049, Shandong Province, China
2
Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, C
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