Determination of aflatoxin M 1 and deoxynivalenol biomarkers in infants and children urines from Bangladesh
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TOXICOKINETICS AND METABOLISM
Determination of aflatoxin M1 and deoxynivalenol biomarkers in infants and children urines from Bangladesh Nurshad Ali1 · M. Manirujjaman2 · Sohel Rana3 · Gisela H. Degen4 Received: 15 June 2020 / Accepted: 14 July 2020 © The Author(s) 2020
Abstract The mycotoxins aflatoxin B 1 (AFB1) and deoxynivalenol (DON) are found worldwide in crops and dietary staples. The prevalence and levels of these contaminants can vary greatly, and data in Bangladeshi food commodities are scarce. To characterize human exposure, we have conducted biomonitoring, analyzing AFM1 (a metabolite of AFB1) and DON levels in urines of adult cohorts in Bangladesh. Yet, AFM1 and DON occurrence has not been studied in the very young population of this country. Thus, the same methods, HPLC-FD for AFM1 and LC–MS/MS for DON analysis, were now applied to determine these biomarkers in urines of infants (n = 49) and young children (n = 105) in Rajshahi and Dhaka district. Overall, AFM1 and DON detection frequency was 43.5% and 33.4%, with 34.7% and 11.5% in infant and 47.6% and 39.4% in children urines, respectively. The mean A FM1 levels in all infants (9.1 ± 14.3, max 55.6 pg/mL) and children (8.8 ± 12.9, max 75.3 pg/mL) were not significantly different. The AFM1 mean level was slightly higher in Dhaka (9.4 ± 12.4) compared to Rajshahi (8.5 ± 13.9 pg/mL) district. The average DON level was about 2-fold higher in infant (3.8 ± 2.9, max 6.8 ng/mL) than children urines (1.6 ± 1.8, max 8.6 ng/mL), and higher in Rajshahi (2.1 ± 2.3 ng/mL) than Dhaka (1.4 ± 1.6 ng/mL) district. The biomarker-based estimated average daily DON intake (29.6 ± 108.3 ng/kg bw in infants and 36.4 ± 81.8 ng/kg bw in children) or the maximum exposure (560 ng/kg bw) do not exceed the current maximum provisional tolerable daily intake value of 1 µg/kg bw for DON, although DON exposure in infants and children is higher than that of Bangladeshi adults. The AFM1 urine levels in young children are somewhat lower than those found previously in adult cohorts in Bangladesh, but the frequent detection of this biomarker for AFB1 exposure raises further concerns, also for this vulnerable part of the population. Therefore, continuous surveillance for aflatoxins in Bangladeshi food commodities is clearly required, first to identify major sources of intake and then to reduce exposure. Keywords Aflatoxins; deoxynivalenol; biomarkers · Infant and children · Bangladesh
2
* Gisela H. Degen [email protected]
Department of Biochemistry, Gonoshasthaya Samaj Vittik Medical College, Gono University, Savar, Dhaka 1344, Bangladesh
3
M. Manirujjaman [email protected]
Department of Veterinary and Animal Science, Rajshahi University, Rajshahi 6205, Bangladesh
4
Leibniz-Research Centre for Working Environment and Human Factors (IfADo) at the TU Dortmund, Ardeystr. 67, 44139 Dortmund, Germany
* Nurshad Ali [email protected]
Sohel Rana [email protected] 1
Department of Biochemistry and Molecular Biology, Shahjalal University of Science a
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