Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension
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ORIGINAL ARTICLE
Determination of UCP1 expression in subcutaneous and perirenal adipose tissues of patients with hypertension Xueqin Li1,2 • Juan Liu1 • Gongcheng Wang3 • Jing Yu1 • Yunlu Sheng1 • Chen Wang1 Yifan Lv1 • Shan Lv1 • Hanmei Qi1 • Wenjuan Di1 • Changjun Yin4 • Guoxian Ding1
•
Received: 4 January 2015 / Accepted: 3 March 2015 Ó Springer Science+Business Media New York 2015
Abstract The objective of this study is to determine the property of human perirenal adipose tissue (PAT) and assess the adipose property of PAT in hypertension. Ninety-four patients, including 64 normotensive patients (T-NP) and 30 hypertensive patients (HP), who underwent renal surgery were included. Expression analysis was performed using quantitative real-time polymerase chain reaction, Western blot, and immunohistochemistry in PAT and back subcutaneous adipose tissue (bSAT) depots. Compared with bSAT, PAT adipocytes were smaller, and the expressions of uncoupling protein-1 (UCP1) mRNA and protein were markedly higher, while the mRNA expressions of markers for classic beige and white adipocytes
were lower in PAT. Immunohistochemistry analysis showed more multilocular UCP1-positive adipocytes in PAT than in bSAT. UCP1 expressions were lower in PAT in HP than in the T-NP or age- and body mass indexmatched NP groups. Bigger unilocular adipocytes with less UCP1 staining in PAT were detected in HP than in NP group, although no such difference was observed in bSAT. PAT acts as a brown-like fat. UCP1 expression of PAT was lower in HP than in normotensive patients. UCP1 expression of PAT may serve as a protective indicator for hypertension. Keywords Uncoupling protein 1 Perirenal adipose tissue Hypertension
Xueqin Li and Juan Liu have contributed equally to this work.
Electronic supplementary material The online version of this article (doi:10.1007/s12020-015-0572-3) contains supplementary material, which is available to authorized users. & Changjun Yin [email protected] & Guoxian Ding [email protected] 1
Department of Geratology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, People’s Republic of China
2
Department of Geratology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing Road West, Huai’an 223300, Jiangsu, People’s Republic of China
3
Departments of Urology, Huai’an First People’s Hospital, Nanjing Medical University, 6 Beijing Road West, Huai’an 223300, Jiangsu, People’s Republic of China
4
Department of Urology, The First Hospital Affiliated to Nanjing Medical University, 300 Guangzhou Road, Nanjing 210029, People’s Republic of China
Introduction Adipose tissue is a major metabolic organ, and it has been traditionally classified as either brown adipose tissue (BAT) or white adipose tissue (WAT). Excess fat accumulation is causally linked with various metabolic risk factors including type 2 diabetes, hypertension, and dyslipidemia, which leads to development of cardiovascular diseases [1, 2]. Human fat mass, especially abdominal fat
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