MiRNA expression in the cartilage of patients with osteoarthritis
- PDF / 900,793 Bytes
- 7 Pages / 595.276 x 790.866 pts Page_size
- 107 Downloads / 214 Views
RESEARCH ARTICLE
Open Access
MiRNA expression in the cartilage of patients with osteoarthritis Marta Kopańska1,2†, Dariusz Szala3†, Joanna Czech1,2, Natalia Gabło1,2, Krzysztof Gargasz4, Mateusz Trzeciak3, Izabela Zawlik1,2 and Sławomir Snela3*
Abstract Background: Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. Methods: Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. Results: Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). Conclusions: These findings may contribute to disease prevention and the development of therapeutic targets for OA. Keywords: Osteoarthritis, Cartilage, miRNA, Expression, Pathogenesis, Patients
Background Osteoarthritis (OA) is a complex multifactorial disease caused by genetic, mechanical, and environmental factors, and it is the most prevalent disease of the articulating joints. Many environmental factors, such as hormones, diet, infections, injury, alcohol intake, and exposure to tobacco smoke, are associated with an increased risk of OA. Exposure of genetically susceptible individuals to such environmental factors may promote disease development [1]. Environmental factors induce epigenetic mechanisms that regulate genomic activity independently of changes in the DNA sequence and alter the expression of genes involved in disease development [2]. The three pillars of epigenetic regulation are DNA methylation, histone modification, and non-coding RNA species, including miRNA. The epigenetic mechanisms involved in the pathogenesis of OA are not understood [3]. Whether aberrant miRNA expression is associated with OA development is unclear. Many recent studies suggested that epigenetic events play a critical role in OA progression. Moreover, changes in gene * Correspondence: [email protected] † Equal contributors 3 Clinical Orthopedics and Traumatology Department in Clinical Hospital 2, 35-301 Rzeszow, Poland Full list of author information is available at the end of the article
expression have been observed in diseased cartilage [4]. Epigenetic mechanisms occur through miRNA expression. miRNAs comprise a large family of single-stranded, small, non-coding RNAs with a sequence length of 19 to 23 nucleotides. These molecules typically bind to the 3′ untranslated region of their target messenger RNAs (mRNAs) and repress protein expression by inhibiting mRNA translation and/or destabilizing mRNA. Micro RNAs are implicated in many cellular functions, such as apoptosis, lipid metabolism, malign
Data Loading...
