Development of a Micro fluidic Nanoscale Protein Sensor Device for Improving Vascular Surgical Outcomes
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Development of a Micro fluidic Nanoscale Protein Sensor Device for Improving Vascular Surgical Outcomes. Shalini Prasad Department of Electrical Engineering, Portland State University, Portland, OR-97201 Abstract: The normal physiologic response of vascular surgery is one of acute and intense inflammation and thrombosis. Research to date on improving outcomes after surgery has focused on drugs known to be active with the adrenergic system more specifically statins. Till date there has been no correlation between inflammation post surgery and the effect of perioperative antiinflammatory drugs on improving surgical outcomes. The goal of this research is to characterize the role perioperative inflammation and thrombosis plays in determining outcomes after surgery. The goal of this particular application is to identify two specific pro-inflammatory protein markers namely C-reactive protein (CRP) and Myloperoxidase (MPO) associated with thrombosis from a wide range of serum samples. This is achieved by integrating the principles of fabrication associated with micro and nanotechnology to develop a sensor device comprising of nano ordered porous alumina membrane embedded in an elastomer (polydimethylsiloxane, PDMS) micro fluidic base. The nano porous membrane is selectively functionalized by a two level masking technique to the binding of CRP and MPO in selected areas. The binding event results in an electrochemical reaction that in turn produces a change in the surface charge. This in turn produces a measurable change to the electrical voltage. This is measured in an in-situ, non-invasive manner from selectively metalized areas of the nano membrane. Energy density analysis yielded unique electrical identifiers for each of the protein markers. Detection sensitivity of the order of 10 ppm was observed. 1. Introduction: In recent years, rapid advancements have been made in the biomedical applications of micro- and nanotechnology 1, 2. The focus of such technology to date has primarily been on invitro platform based analysis and diagnostic tools. Over the past few years applications in the sensing domain have gained attention3. The long-term integration of organic material such as cells, proteins and nucleic acids with inorganic materials provides the basis for novel sensing platforms. This work focuses on the ability to isolate and detect specific pro-inflammatory protein markers from serum samples on nanoporous alumina based micro environments. Micro-Electro-Mechanical Systems technology, also known as MEMS, refers to the fabrication of devices with dimensions on the micrometer scale. The most essential elements of MEMS consist of miniaturized, highly repeatable, and precise structures that can be stationary or moving. These structures are created via fabrication processes and equipment developed for the integrated circuit (IC) industry4, 5. Typically, micro fabrication has a limit of resolution on the order of microns. However, specialized techniques involving electrochemistry are used to create features in
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