Development of biocompatible DES/NADES as co-solvents for efficient biosynthesis of chiral alcohols
- PDF / 1,478,464 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 37 Downloads / 148 Views
RESEARCH PAPER
Development of biocompatible DES/NADES as co‑solvents for efficient biosynthesis of chiral alcohols Nana Xia1 · Lilu Xiong1 · Shunde Bi1 · Feng Qian1 · Pu Wang1 Received: 8 February 2020 / Accepted: 1 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The novel deep eutectic solvents (DESs) and natural deep eutectic solvents (NADESs) were designed and synthesized by cell protective components, in which the compounds were derived from natural alternative sources. The performances of designed DESs/NADESs as co-solvent were investigated in asymmetric reduction catalyzed by microbial cells. The DESs/ NADESs synthesized by three different types of hydrogen bond receptor (betaine, l-proline and l-carnitine) conferred an advantage over conventional choline chloride-based DESs/NADESs and aqueous buffer system, with regard to efficient bioproduction of (R)-1-[4-(trifluoromethyl)phenyl]ethanol by recombinant Escherichia coli cells. TEM images exhibited that the cell membrane integrity during exposure to the developed NADESs was better than that after treatment with choline chloride-based NADES, which accounted for enhanced catalytic efficiency. This bioprocess was also feasible at 500 mL preparation scale with 92.4% yield under 400 mM substrate loading. To broaden the applicability of three types of DES/ NADESs that increased catalytic efficiency in the process of E. coli-mediated reduction, the production of various chiral alcohols in developed reaction media were further examined, with some positive results. It was also found that lysine-based NADES could even reverse the enantioselectivity of biocatalyst at high water content in the reaction medium. These findings may aid in the development of novel DESs/NADESs for biocatalysis. Keywords Deep eutectic solvents (DES) · Natural deep eutectic solvents (NADES) · Biocatalysis · Bioreduction · Co-solvent
Introduction The biocatalytic production of pharmaceuticals and pharmaceutical intermediates has become one of the best solutions for achieving “green chemistry” gist, and the essential method for acquiring chiral alcohols because it is an eco-friendly and more sustainable manufacturing process [1–3]. Biocatalysis can be accomplished using the whole cell or the isolated enzymes [3, 4]. Comparatively, the wholecell catalysis is a cost-effective approach, which avoids the extra supplement of coenzymes and provides more stable Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00449-020-02387-5) contains supplementary material, which is available to authorized users. * Pu Wang [email protected] 1
College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, People’s Republic of China
environments for enzymatic reaction [5]. However, wholecell catalysts sometimes exhibit some limitations including low solubilities of the synthetic substances in aqueous medium, as well as low volumetric and catalyst productivities [6]. An effective way for improving the bioc
Data Loading...
