Dielectrophoretic Trapping of Neuronal Cells
In this section, it is shown that dielectrophoresis can effectively be used to trap cortical rat neurons. The trapping process was found to depend on amplitude and frequency in a nonlinear way. Several aspects were theoretically and experimentally investi
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Vol. 173
Editors F. Beck, Melbourne B. Christ, Freiburg W. Kriz, Heidelberg W. Kummer, Gießen E. Marani, Leiden R. Putz, Mnchen Y. Sano, Kyoto T. H. Schiebler, Wrzburg K. Zilles, Dsseldorf
Springer-Verlag Berlin Heidelberg GmbH
T. Heida
Electric Field-Induced Effects on Neuronal Cell Biology Accompanying Dielectrophoretic Trapping
With 46 Figures and 7 Tables
BD
Dr. Tjitske Heida University of Twente Faculty of Electrical Engineering Mathematics and Computer Science Laboratory of Measurement and Instrumentation Laboratory of Biomedical Engineering P.O. Box 217 7500 AE Enschede The Netherlands e-mail: [email protected]
ISSN 0301-5556 ISBN 978-3-540-00637-4 ISBN 978-3-642-55469-8 (eBook) DOI 10.1007/978-3-642-55469-8 Library of Congress Cataloging-in-Publication Data Heida, T. (Tjitske), 1972– Electric field-induced effects on neuronal cell biology accompanying dielectrophoretic trapping / T. Heida p. cm. – (Advances in anatomy, embryoloy, and cell biology, ISSN 0301-5556 ; v. 173) Includes bibliographical references and index. 1. Neurons. 2. Dielectrophoresis. 3. Nerves–Electric properties. 4. Microelectrodes. I. Title. II. Series. This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law. http://www.springer.de Springer-Verlag Berlin Heidelberg 2003 Originally published by Springer-Verlag Berlin Heidelberg New York in 2003 The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher cannot guarantee the accuracy of any information about dosage and application contained in this book. In every individual case the user must check such information by consulting the relevant literature. Typesetting: Strtz, Wrzburg Printed on acid-free paper
27/3150Ag – 5 4 3 2 1 0
Contents
1 1.1 1.1.1 1.1.2 1.2 1.2.1 1.2.2 1.3 1.3.1 1.3.2 1.4 1.4.1 1.4.2 1.5
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Neuro-Electronic Interfacing . . . . . . . . . . . . . . . . . . . . . . Nervous System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Restoring Neuronal Functions. . . . . . . . . . . . . . . . . . . . . Culturing Neuronal Cells. . . . . . . . . . . . . . . . . . . . . . . . . . Dissociation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Culturing Conditions . .
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