Differentially expressed proteins in the development of protocorm-like bodies of Cattleya tigrina A. Richard
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ORIGINAL ARTICLE
Differentially expressed proteins in the development of protocorm‑like bodies of Cattleya tigrina A. Richard Daniela De Conti1 · Jenny Paola Corredor‑Prado2 · Jessica Cavalheiro Ferreira Bueno3 · Gabriela Claudia Cangahuala‑Inocente3 · Miguel Pedro Guerra3,4 · Rosete Pescador3 Received: 4 February 2019 / Revised: 9 November 2020 / Accepted: 20 November 2020 / Published online: 27 November 2020 © Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2020
Abstract Cattleya tigrina is an endangered Brazilian native orchid with ornamental potential. Biotechnological tools based on tissue culture techniques are effective for its mass propagation and conservation. Protocorm-like bodies (PLBs) were induced from leaves in culture medium supplemented with plant growth regulators, and generated complete in vitro plants. To deepen on the mechanism underlying this morphogenetic route, this study is aimed to identify and characterize differentially expressed proteins during C. tigrina PLBs development. Leaves were inoculated in in culture medium Murashige and Skoog (MS) supplemented with 9 μM Thidiazuron, and PLBs t were collected after 30, 60 and 100 days in culture. Proteomic analysis performed by two-dimensional electrophoresis and MALDI-TOF mass spectrometry detected 122, 132 and 447 protein after 30, 60 and 100 days, respectively. During PLBs development, 36 proteins were differentially expressed. Proteins related to energy and carbohydrate metabolism, cell proliferation, protein processing, and secondary metabolism and phytohormones were assessed showing their relevance in the process of development of PLBs. Many proteins related to metabolic and energy processes were identified after 60 and 100 days in culture, suggesting their role in which is cell division and differentiation. Enolase was only present after 100 days in culture, thus being a candidate as a molecular marker in this developmental phase. Keywords 2-DE · Orchid · Protocorm-like bodies · Proteomic
Introduction The Atlantic Forest, an hotspot of biodiversity has its domain in the Northeast, Southeast and South of Brazil, and harbor Cattleya tigrina, an epiphytic or rupicolous species Communicated by S. Abe. * Daniela De Conti [email protected] 1
Faculdade do Vale do Araranguá, Av. Getúlio Vargas, 415‑Centro, Araranguá, Santa Catarina 88900‑000, Brazil
2
Departament of Biology and Chemistry, Universidade de Sucre, Barrio Puerta Roja, 28 # 5‑267, Ciudad Sincelejo, Estado Sucre, Colombia
3
Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina, Admar Gonzaga, Road, 1346, Itacorubi, Florianópolis, Santa Catarina 88034‑001, Brazil
4
Graduate Program in Agricultural and Natural Ecosystems, Federal University of Santa Catarina, Curitibanos Campus, Ulysses Gaboardi Road, Km 3, Curitibanos, Santa Catarina 89520‑000, Brazil
endemic to this biome (Barros et al. 2016). The decrease in its population, due to the drastic reduction of its habitat and its indiscriminate c
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