Comparative proteomic analysis of differentially expressed proteins related to phloem and xylem development in rubber tr
- PDF / 1,949,835 Bytes
- 19 Pages / 595.276 x 790.866 pts Page_size
- 64 Downloads / 197 Views
ORIGINAL ARTICLE
Comparative proteomic analysis of differentially expressed proteins related to phloem and xylem development in rubber tree (Hevea brasiliensis) Piyawadee Prasongsansuk1,2 · Tipparat Thiangtrongjit3 · Kanlaya Nirapathpongporn4 · Unchera Viboonjun5 · Panida Kongsawadworakul5 · Onrapak Reamtong3 · Jarunya Narangajavana1,2 Received: 4 February 2020 / Accepted: 3 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message The proteomic analysis of vascular tissues in rubber tree reveals differentially expressed proteins related to laticifer differentiation in mature phloem and secondary cell wall formation in mature xylem for latex/wood-yield improvement. Abstract Rubber tree (Hevea brasiliensis) is a latex-producing plant that has worldwide economic importance for the rubber and wood industries. Natural rubber latex is produced from laticiferous vessels (laticifers) located in the secondary phloem tissue of rubber trees. Improving latex production from rubber tree clones by studying the molecular genetics of vascular development has received much attention, but less information has been obtained from proteomic approaches. This study performed a comparative proteomic analysis of the vascular tissues in high latex-yield and high wood-yield rubber tree clones. One primary vascular tissue (newly developed stem) and two secondary vascular tissues (mature phloem/laticifers and mature xylem/wood) were investigated by qualitative/quantitative proteomic analysis using GeLC-MS/MS. The differentially expressed proteins (DEPs) in the specific vascular tissues were analyzed, and the protein functions in the biological processes related to vascular development and to the specific characteristics of each clone were clarified. The predicted protein–protein interaction network and GO annotation revealed DEPs related to photosynthesis, carbohydrate metabolism, energy production and jasmonic acid-responsive proteins involved in positive regulation of laticifer differentiation in mature phloem of the high latex-yield clone, while DEPs related to cell cytoskeleton maintenance, secondary cell wall components and auxin-, brassinosteroid-, abscisic acid-responsive proteins involved in xylem differentiation were abundant in mature xylem of the high wood-yield clone. This is the first report to demonstrate the correlation and functions of DEPs in phloem/ laticifers and xylem/wood differentiation in rubber trees. The regulation of vascular development could be useful in improvement of latex and wood yields from rubber trees. Keywords Laticifer · Rubber tree · Vascular tissue · Vascular development-related protein · Wood
Communicated by DeMicco. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00468-020-02019-1) contains supplementary material, which is available to authorized users. * Jarunya Narangajavana [email protected] 1
Department of Biotechnology, Faculty of Science, Mahidol University, Phayathai, Rama 6 Rd., Rajthewee, B
Data Loading...
